Burkholderia cepacia, growth

Hur H-G, LM Newman, LP Wackett, MJ Sadowsky (1997) Toluene 2-monooxygenase-dependent growth of Burkholderia cepacia G4/PR1 on diethyl ether. Appl Environ Microbiol 63 1606-1609. 

Mars, A.E., J. Houwing, J. Dfolfing, and D.B. Janssen. 1996 Degradation of toluene and trichloroethylene by Burkholderia cepacia G4 in growth-limited fed-batch culture. Appl. Environ. Microbiol. 62 886-891. 

C. necator is the model bacterium for the biosynthesis of PHA. This strain generally initiates the synthesis of PHA when either nitrogen or phosphorous is limited during growth (Kahar et al. 2004). A similar phenomenon occurs in several other PHA producers including Burkholderia cepacia (Zazali and Tan 2005 Mitomo et al. 1999), Pseudomonas sp. (Choi et al. 2003), and A. hydrophila... 

Culture and fermentation conditions. PHA production experiments were conducted in shake-flasks using cultures of Burkholderia cepacia, obtained from the American Type Culture Collection (ATCC No. 17759, Bethesda, MD). Cultures of B. cepacia were maintained at 25 C and transferred weekly on agar slants containing xylose (2.2 % w/v) and levulinic acid (0.3 % w/v) as carbon sources. Levulinic acid was added to cultures as a concentrated solution (pH adjusted to 7.2 via NaOH prior to sterilization). Inocula were prepared as 500-1000 ml cultures in 2,800 ml Fernbach flasks containing 2.2 % (w/v) xylose and 0.07 % (w/v) levulinic acid, incubated at 28 C and 150 rpm for 72 hours. For PHA production, shake-flasks were inoculated with a 5 % (v/v) aliquot of the above-described seed culture, incubated at 28 C and 150 rpm for 20 hours, and were then supplemented with a second dose (0-0.5 % w/v) of levulinic acid. Flasks were subsequently incubated for an additional 62-115 hours of growth in order to assess time-related differences in polymer production, and then harvested for biomass and PHA extraction, as previously described (20),... 

Miller S, LiPuma J, Parke J (2002) Culttne-based and non-growth-dependent detection of the Burkholderia cepacia complex in soil environments. Appl Environ Microbiol 68(8) 3750-3758 Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Eeatures of pnnnising teehnologies for pretreatment of lignocellulosic biomass. Bioresonr Technol 96(6) 673-686... 

Mars, A.E, Houwing, I, Dolfing, J. Janssen, D.B. 1996. Fed-Batch Culture by Burkholderia cepacia G4 in growth-Umited degradation of toluene and trichloroethylene. Appl Environ. Microbiol. 62(3) 886. 

Although toluene degradation in pseudomonads may be induced by growth with the substrate or closely related aromatic compounds, it may also be induced by exposure to apparently unrelated substrates (1) by trichloro-ethene in a strain of P. putida (Heald and Jenkins 1994) and (2) in P. mendocina strain KR1 by trichloroethene, pentane, and hexane, although not in Burkholderia (Pseudomonas) cepacia, or P putida strain FI (McClay et al. 1995). 

The isolation of Burkholderia sp TPI 064 (recently B. cepacia) was recently discovered. This bacterium is capable of accumulating polymers with unsaturated monomers from carbohydrates, i.e. without substrate supply with chemical structure directly related to the product. This strain, as well as ten other strains isolated from soil and poly(HB-co-HPE) producers, were compared with conversion factors from substrates to polymer (y P/S). The best strains were evaluated with respect to maximum specific growth rate values (/ixmax) and for pentenoic acid conversion into HPE units. ... 

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