5-bromo deoxyuridine

CflHuBrNjOij 5-Bromo-2 -deoxyuridine (monoclinic form) BROXURIO 31 369... 

In 1958 Taylor demonstrated SCEs, using autoradiographic techniques to detect the disposition or labeled DNA following incorporation of [3H]-thymidine. 5-Bromo-2 -deoxyuridine (drdU) has now replaced [3H]-thymidine and various staining methods have been used to show the differential incorporation of BrdU between sister chromatids fluorescent Hoechst 33258 (Latt, 1973) combined fluorescent... 

Fig. 8.9. Activation of basic ester prodrugs of 5-bromo-2 -deoxyuridine (8.132) by cyclization of the pro-moiety (Reaction a) and by enzymatic hydrolysis (Reaction b) [170]...

The development of monoclonal antibodies which recognize halogenated pyrimidines such as 5-bromo-2-deoxyuridine (BrdU) incorporated into DNA (1) and of flow cytometric (FCM) techniques to simultaneously measure BrdU uptake and total DNA content (2) have led to a renaissance in cell kinetic studies. The speed and quantitative power of the flow cytometer, m conjunction with the specificity and sensitivity of monoclonal antibody techniques, provide the basis for the adoption and success of the BrdU technique in experimental and clinical investigations. 

Bromo-2-deoxyuridine (BUdR) Dissolve 10 mg/mL in distilled water. Sterilize by filtration with a 0 2-pm filter. Store m aliquots at -20°C... 

Wojcik A, Bochenek A, Lankoff A, Risowska A, Padjas A, von Sonntag C, Obe G (2005) DNA interstrand crosslinks are induced in cells prelabelled with 5-bromo-2 -deoxyuridine and exposed to UVC radiation, in preparation... 

Wojcik A, Opalka B, Obe G (1999) Analysis of inversions and sister chromatide exchanges in chromosome 3 of human lymphocytes exposed to X-rays. Mutagenesis 14 633-637 Wojcik A, von Sonntag C, Obe G (2003) Application of the biotin-dUTP chromosome-labelling tech-nigue to study the role of 5-bromo-2 -deoxyuridine in the formation of UV-induced sister chromatid exchanges in CHO cells. J Photochem Photobiol B Biol 69 139-144 Wu JC, Kozarich JW, Stubbe J (1983) The mechanism of free base formation from DNA by bleomycin. J Biol Chem 258 4694-4697... 

BrdU (5-bromo-2 -deoxyuridine) was purchased from Sigma-Aldrich Japan (Tokyo, Japan), and was dissolved in saline with 0.007 N NaOH at a concentration of up to 10 mg/ml. BrdU was intravenously injected in the saphenous vein as each injection was at a dose of 100 mg/kg. The neonatal monkey received two injections one on the day of birth, and one on PI4. Of the 28 adult monkeys, 22 received 5 injections on five consecutive days, and were sacrificed on various periods after the last BrdU injection. Based on these periods, two monkey groups emerged a group with short-term survival after BrdU, the monkeys within which... 

Following the addition of fresh growth medium and BrdU (5-bromo-2 -deoxyuridine), cells incubated for 20 hours. 

A more recently developed non-radioactive test uses the incorporation of 5-bromo-2 -deoxyuridine as a measure of cell proliferation (Takeyoshi et al. 2004). 

SYNS BDU 5-BDU BROMODEOXYURIDINE 5-BROMODEOXYURIDINE 5-BROMO-2-DEOXYURIDINE 5-BROMODESOXYURIDINE BROMOURACILDEOXYRIBOSIDE 5-BROMOURACIL DEOXYRIBOSIDE 5-BROMOURACIL-2-DEOXYRIBOSIDE BROXURIDINE BRUDR BUDR 5-BUDR... 

Bromo-2-deoxyuridine This can be obtained from Sigma Chemical Co. (Poole, UK) for experimental studies. A preparadon suitable for in vivo use in humans can be obtained from the Invesdgadonal Drugs Branch of the Nadonal Cancer Insdtute, Bethesda, MD. 

Complete Medium (CM) 76mLofRPMI 1640,20mLoffetal bovine serum, 2 mL of phytohemagglutinin (M FORM), 1 mL of andlxodc/antimycotic solution (lOOX = 10,000 units penicillin, 10,000 tg Streptomycin and 25 pg fungizone) and 1 mL of lOOX Lrglutamine (lOOX = 30 mg/mL). 5 -Bromo-2-deoxyuridine (BUdR) Dissolve 10 mg of BUdR per mL in distilled water. Sterilize by filtration with a 0.2-pm filter. Store aliquots at -20°C. Handle BUdR in subdued light... 

Mild alkaline treatment of 5-bromo-2 -deoxyuridine 1523 results in a ring contraction of the pyrimidine to form the imidazolinone nucleoside 1524. The a-anomer was isolated after chromatographic purification (Scheme 394) <2005T5081>. 

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