Hemoglobin, bovine

D. Similar methods were used for modification of the enzymes listed in Table II as well as bovine hemoglobin (see Table III). The choice of conditions for the modification reactions (pH, temperature, etc.) was made mainly based on the properties/stability of each protein. Enzymatic activities were measured by previously reported methods (77,27-25). 

Boundary managing, in R D, 21 619-620 Boundary spanning, in R D, 21 619 Bound chloride formation, 10 358 Bound moisture, 9 96 Bourdon tube, 20 647-649 Boussinesq approximation, 11 779 Boutique fuels, 12 419 Bovatec, 20 136 Bovine hemoglobin, 4 125 Bovine insulin, 3 817 Bovine serum albumin (BSA), 20 573 properties of standard, 3 836t Bovine somatotropin (BST), 10 871 Bovine spongiform encephalitis/... 

Substrate Peanut Arachin gg Albumin Bovine Serum Albumin Bovine Hemoglobin B-Lacto- globulin Wheat Gliadin Yam Protein... 

Fronticelli C, Sato T, Orth C, et al. Bovine hemoglobin as a potential source of hemoglobin-based oxygen carriers crosslinking with bis(2,3-dibromosalycyl)fu-marate. Biochim Biophys Acta 1986 874 76. 

Sakai H, Masada Y, Takeoka S, et al. Characteristics of bovine hemoglobin as a potential source of hemoglobin-vesicles for an artificial oxygen carrier. J Bio-chem (Tokyo) 2002 131 611. 

Kiehs, K., C. Hansch, and L. Moore, The role of hydrophobic bonding in the binding of organic compounds by bovine hemoglobin , Biochemistry, 5,2602-2605 (1966). 

Dissolve 2.0 g bovine hemoglobin (Sigma) in 35 ml reagent-grade water. Add 36.0 g urea and 16 ml of 0.5 M NaOH. Stir 30 min at room temperature. Add buffer (typically sodium phosphate see appendix 2A), adjust pH to that appropriate for the enzyme system under investigation, and then bring to 100 ml with water. Store up to 5 days at 4°C. 

Schlamowitz, M. and Peterson, L.U. 1959. Studies on the optimum pH for the action of pepsin on native and denatured bovine serum albumin and bovine hemoglobin. J. Biol. Chem. 234 3137-3145. 

Inserted tip, glass chip EOF-hydraulic pump 200 nL/min ESI-TOF Bovine hemoglobin tryptic digest Mixed off-chip, on-chip tryptic digestion Nil 115... 

Hemoglobin and heparin present in blood samples are also PCR inhibitors. In one report, these inhibitors were removed from yeast cells (used as the model for blood cells) after DEP cell retention. The yeast cells were retained by DEP, whereas bovine hemoglobin (1 mg/mL) and heparin (13 pg/mL) were washed away [933],... 

Protein separation has commonly been achieved by isoelectric focusing (IEF). In various reports, IEF separations have been achieved with BSA [1036], bovine hemoglobin [1036], cytochrome c [189], P-lactoglobulin [189], protein markers [975,1037], and bacteria [1038],... 

Tryptic digest of bovine hemoglobin (4 pM) cytochrome c (0.8 pM) Human hemoglobin (normal and sickle cell, 0.24 pM) Mixed off-chip/on-chip tryptic digestion Nil 807... 

After some days in trifluoroacetic acid solution at 5°, the spectra of pepsin and bovine hemoglobin (not shown) exhibit a much more prominent peak in the methionine methyl position. This effect is probably the result of additional rotational freedom, brought about by cleavage of some of the peptide bonds. 

The nitrocellulose membrane is remove from the Genie Electrophoretic Blotter and incubated for 1 hr in buffer A [10 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 2 mM EDTA, 1% (w/v) bovine hemoglobin]. 

The decontamination efficacy of radioprotective agent aminoethylisothiourea and cystamine on rat skin against SM was comparable to standard decontaminants, such as alco-holate, clay, and Fuller s earth (Knezevic and Tadic, 1996). A mixture containing bovine hemoglobin, gelatin, and poi (a Hawaiian foodstuff) on the hydrolytic kinetics of the mustard simulants, 2-chloroethyl ethyl sulfide, 2-chloro-ethyl methyl sulfide and 2-bromoethyl phenyl sulfide, is reported. The kinetic mechanisms and rate constants were dependent upon the mixtures concentration and viscosity (Cemy and Cemy, 1997). 

Bovine serum albumin (BSA) and bovine hemoglobin (BHb) were purchased from Sigma Chemical Co., USA, catalog number A-7906 and H-2500, and no further purification was performed. 

Kasper, S. Grune, F. Walter, M. Amr, N. Erasmi, H. Buzello, W. The effects of increased doses of bovine hemoglobin on hemodynamics and oxygen transport in patients undergoing preoperative hemodilution for elective abdominal aortic surgery. Anesth. Analg. 1998, 87, 284—291. 

Willinger, C.C. Schramek, H. Pfaller, K. Joannidis, M. Deetjen, P. Pfaller, W. Ultrapure polymerized bovine hemoglobin improves structural and functional integrity of the isolated perfused rat kidney. Renal Physiol. Bio-chem. 1995,18, 288-305. 

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