Blunt dissection

Kill immune animals by cervical dislocation or C02 inhalation, test bleed, and open abdominal cavity. Remove spleens or mesenteric nodes by blunt dissection. 

As needed the tumor can be blunt dissected free from the fascia using blunt scissors. 

Obtain fresh human normal-term placentas within 1 h of delivery, and inspect them in a biohazard hood. If necessary, match placenta and cell species. Aseptically, peel the amnion membrane away from the chorion by hand and blunt dissection, and rinse it with PBS supplemented with antibiotics (100 U/ml penicillin, 100 ig/ml streptomycin, 0.5 /xg/ml Fungizone). Use only the outer portions of the amnion (>600 cm, <0.5 mm thick) not directly adjacent to the umbilical cord and decidua transfer them into DMEM with antibiotics. 

Squeeze the scrotal sac to force the testes to the incision, and expose both testes by grasping the associated fat pads with the forceps. Identify the vas deferens and isolate it by blunt dissection. 

Blunt dissection is a fast, elementary, usually nonscarring surgical procedure used to remove rrarts and other epidermal tumors. Unlike ED C and excision, it does not disturb normal tissue. 

Figure 4 - Preparation and insertion of pH probe. 1) The residual 4M KCL solution in the reference chamber is aspirated and replaced with fresh solution. 2 and 3) The sterile probe is calibrated in low (6.86) and high (7.41) standard pH buffers. 4) A 2 cm. incision is made down to the fascia lata in which a 1 cm, incision is made. 5) A subfascial tunnel which will accept the distal 2 cm. of the probe is made with blunt dissection. 6) The probe, the cable of which is attached to a pH meter, is stabilized by closing the fascia and skin over the probe and taping the exposed portion of the probe to the skin. 7) Final position of the probe on the muscle surface. The right angle extension on the exposed plastic cap is useful for securing the probe to the limb and orienting the sensing tip.

The skin is prepared and draped in a sterile fashion, and local anesthesia is injected into the skin and deep soft tissues at the entry site using a 30-gauge needle in the anticipated route. One percent lidocaine mixed with sodium bicarbonate (3 cc 2 cc) minimizes discomfort. Inadequate local anesthesia will result in pain that will awaken the child and make the procedure more difficult and time-consuming. A skin incision is then made with a no. 11 scalpel blade and enlarged with blunt dissection. 

Six to eight weeks after the initial challenge, anaesthetize the rats, test bleed, open the abdomen parallel to the initial incision, and re-immunize using the unchallenged Peyer s patches as recipient for antigen. Three days later kill the animals, remove the mesenteric nodes by blunt dissection, and use for cell fusion. 

Under ether anesthesia, the abdominal skin of a diabetic Wistar rat was closely shaved and swabbed with a 10% Betadine solution. A midline cut was made, and a subcutaneous pocket 3 cm from the incision was created sideways by blunt dissection. An 1/8 piece of the pellet disc containing 20% insulin was inserted and the wound was closed by two Michel clips. The whole procedure required about 3 min, and the animal recovered in less than 1 min thereafter. 

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