Blood interaction with filters

Fig. 3.2 Relationship of the connective tissue stroma to an epithelium. Fibroblasts secrete unique types of laminin proteins that interact with each other and type IV collagen, to form a basal lamina (yellow) that is tightly attached to the connective tissue (stromal) cells and also to the associated muscle or epithelial cell. The kidney glomerulus is a specialized tissue in which the stroma is absent and endothelial cells and epithelial cells are separated by the basal lamina which filters the blood as the first step in urine collection. The attachment of an epithelium to stromal collagen is discussed in detail in Chap. 5 (Fig. 19-55 in The Molecular Biology of the Cell. B. Alberts et al., 4th Ed. 2002. Garland Science, Taylor Francis Group, NY)...

The change in biological response of the adsorbed fibrinogen molecule (conversion), is also noticeable with platelet adhesion studies. In confirmation of earlier studies of Zucker and Vroman (5), we found that, usually, less platelets adhered to areas of glass slides exposed to platelet-poor plasma for 3 min than areas exposed for 3 s. When, however, a gel-filtered platelet suspension was used in place of platelet-rich plasma, a dramatic difference in the number of platelets attached to the surface previously exposed to platelet-poor plasma for 3 s or 3 min occurred. Therefore, this more reproducible protocol was used to study not only the adhesion of platelets onto artificial surfaces but also as a probe of conversion. For this purpose we chose a series of block copolymers with controllable domain morphology (phase separation on a molecular scale) and different surface energies (wettability). Previous studies have shown that the degree of phase separation influences the interactions with blood components (6,7). 

The kidneys play important roles in regulating acid-base balance, water and electrolyte balance, blood volume, and blood pressure by interacting with blood hormones. For example, any major change of plasma osmolality is detected by the hypothalamus, which relays messages to the posterior pituitary gland, which then alters the secretion of antidiuretic hormones. Some metabolites (e.g., amino acids and glucose, which are filtered by the glomeruli) are reabsorbed by the tubules and conserved for recirculation. 

Compound is immobilized on a cellulose-based fiber substrate." The fiber substrate filters the blood so that only the serum interacts with 1, The higher the concentration of Na" " in serum, the higher the fraction of 1 Na" ". This form is fluorescent, whereas 1 itself is significantly less fluorescent. The fluorescence intensity then produces, with appropriate calibration, the blood Na+ level. 

The lithium distribution in red blood cells has been investigated using Li NMR spectroscopy. Li NMR imaging has been applied to lithium in the brain and muscle of rats. The dipolar interaction of Na with hydrogen nuclei in glycerol solution has been measured.The phase diagram of a lyotropic mixture sodium bis(2-ethylhexyl) sulfosuccinate/dodecanol/water has been studied using Na NMR spectroscopy. Multiple-quantum filtered and Na NMR... 

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