Blood cell membrane, interaction

N. Shaklai, J. Yguerabide, and H. M. Ranney, Interaction ofhemoglobin with red blood cell membranes as shown by a fluorescent chromophore, Biochemistry 16, 5585-5592 (1977). 

Several ILC studies covering drug interaction with liposomes and, correspondingly, proteoliposomes, cytoskeleton-depleted red blood cell membrane vesicles, red blood cell membranes, or red cells and ghosts have been reported (7,8,21-28,40,76,91,92,94). The log Ks values for interaction of... 

The unsaturated phospholipid from soybean lecithin also shows a similar effect, while the unsaturated phospholipids from red blood cell membranes, although showing a slight effect of cholesterol interaction, still show a prominent polymethylene peak in the high resolution spectrum. 

DSC has been used to study the individual protein components of biological membranes of relatively simply protein composition and the interaction of several of these components with lipids and with other proteins. The red blood cell membrane, which has been most intensively studied, exhibits five discrete protein transitions, each of which has been assigned to a specific membrane protein. The response of each of these thermal transitions to variations in temperature and pH as well as to treatment with proteases, phospholipases, specific labelling reagents, and modifiers and inhibitors of selected membrane activities, has provided much useful information on the interactions and functions of these components in the intact erythrocyte membrane (46-49). Similar approaches have been applied to the bovine rod outer segment membrane (50) and to the spinach chloroplast thylakoid membrane (51). 

The principal toxicological effect observed upon overexposure to EGBE is the destruction of red blood cells (i.e., hemolysis). BAA, the predominant oxidative metabolite of EGBE, appears responsible for this hemolytic activity. It has been speculated that BAA may interact with red blood cell membranes disrupting erythrocyte osmotic balance, leading to cellular swelling, loss of deformability, and eventually... 

In this paper, we examine the Interactions of pyran copolymer with model biomembranes of two kinds 1) the human red blood cell membrane (or red cell "ghost") and 11) multilamellar suspensions (liposomes) of dlpalmltoylphosphatldylchollne (DFPC), a pure synthetic phospholipid. Each of these systems offers advantages In studies of polymer-cell surface Interaction The red cell membrane, idille complex. Is still the most readily Isolated and best understood of the membranes of nonnal human cells, and Its molecular architecture Is, In a general way at least, typical of such membranes. The pure phospholipids provide a much simpler biomembrane model, with the prospect of yielding more complete Interpretation of experimental observations. 

Zhao Y et al (2011) Interaction of mesoporous silica nanoparticles with humanred blood cell membranes size and surface effects. ACS Ntmo 5(2) 1366-1375... 

The properties of monolayers of the cyclodepsipeptide, valinomydn (Fig. 4.12) have been the subject of recent investigations. Ries and Swift [105] have determined a molecular area of 3.70 nm from extrapolations of pressure-area isotherms for monolayers of valinomydn from which they infer a horizontal orientation of molecules within the monolayer. Mixtures of valinomydn and cholesterol (which has a vertical orientation in monolayers) have been investigated in view of their similarity to biological membranes and the possibility of their use as models for naturally occurring membranes. Monolayers of valinomydn are also of interest because of the ability of valinomycin to stimulate the transport of ions across mitochondrial and red blood cell membranes [106]. Several workers have studied the interaction of electrolytes with valinomycin monolayers [106-108]. These studies have shown a specific interaction of... 

Brody AR, George G, Hill LH. Interactions of chrysotile and crocidolite asbestos with red blood cell membranes. Lab Invest 1983 49 468—475. 

The interaction of irritating substance with the cell membrane can also be measured if red blood cells are used. With a photometric determination the hemolysis (L f) and denaturation (D f) is measured. The ratio LID is an expression for the dermatological compatibility. Low LID figures describe a certain irritation, high LID figures describe a substance as mild. Results found for the... 

Figure 52-4. Diagrammatic representation of the interaction of cytoskeletal proteins with each other and with certain integral proteins of the membrane of the red blood cell. (Reproduced, with permission, from Beck WS, Tepper Rl Hemolytic anemias III membrane disorders. In Hematology, 5th ed. Beck WS [editor]. The MIT Press, 1991.)...

Each cell is surrounded by a plasma membrane that separates the cytoplasmic contents of the cell, or the intracellular fluid, from the fluid outside the cell, the extracellular fluid. An important homeostatic function of this plasma membrane is to serve as a permeability barrier that insulates or protects the cytoplasm from immediate changes in the surrounding environment. Furthermore, it allows the cell to maintain a cytoplasmic composition very different from that of the extracellular fluid the functions of neurons and muscle cells depend on this difference. The plasma membrane also contains many enzymes and other components such as antigens and receptors that allow cells to interact with other cells, neurotransmitters, blood-borne substances such as hormones, and various other chemical substances, such as drugs. 

A highly stable and shielded polyplex should circulate in the blood stream without undesired interactions until it reaches the target cell. At that location, specific interactions with the cell surface should trigger intracellular uptake. While lipid membrane interaction is undesired at the cell surface, it should happen subsequently within the endosomal vesicle and mediate polyplex delivery into the cytosol. During or after intracellular transport to the site of action, the polyplex stability should be weakened to an extent that the nucleic acid is accessible to exert its function. 

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