Bioluminescent assays

Blum L.J., Gautier S.M., Coulet P.R., Continuous-flow bioluminescent assay of NADH using a fiber-optic sensor, Anal. Chim. Acta 1989 226 331. 

Wildish DJ (1976) Determination of adenosine 5 -triphosphate in estuarine water and sediments by firefly bioluminescence assay. Technical report of the fisheries maritime service research division, no. 649... 

The possibility of isolating the components of the two above-reported coupled reactions offered a new analytical way to determine NADH, FMN, aldehydes, or oxygen. Methods based on NAD(P)H determination have been available for some time and NAD(H)-, NADP(H)-, NAD(P)-dependent enzymes and their substrates were measured by using bioluminescent assays. The high redox potential of the couple NAD+/NADH tended to limit the applications of dehydrogenases in coupled assay, as equilibrium does not favor NADH formation. Moreover, the various reagents are not all perfectly stable in all conditions. Examples of the enzymes and substrates determined by using the bacterial luciferase and the NAD(P)H FMN oxidoreductase, also coupled to other enzymes, are listed in Table 5. 

Procedure 8.7 Bioluminescence assay of FMN using bacterial luciferase (EC... 

Kurkijarvi et al. were the first to demonstrate the feasibility of seg-mented-flow bioluminescence assays by use of a bioreactor packed with bacterial bioluminescent enzymes immobilized on Sepharose 4B [60]. The packed glass colunrn used was placed in front of the photomultiplier tube of a luminometer. The luminescence signal obtained was linearly related to the NADH concentration from 1 pmol to 10 nmol for sample volumes of 2-20 pL. In the region of 400 NADH assays could be performed with a single enzyme column, with no appreciable change in sensitivity or accuracy. However, problems arising from packing or disruption of the matrix were encountered after 4 days of intensive use. 

D.C. VELLOM and L.J. KRICKA, "Continuous-Flow Bioluminescence Assays Employing Sepharose-Immobilized En2ymes", in "Methods in Enzymology", vol. 133 (M.A. DELUCA and W.D. McELROY, Eds.) Academic Press, New York, 1986, pp 229-237. 

Elmore, E. Fitzgerald, M.P. (1990) Evaluation of the bioluminescence assays as screens for genotoxic chenticals. In Mendelsohn, M.L. Albertini, R.J., eds, Mutation and the Environment, Part D, New York, Wiley-Liss, pp. 379-387... 

Photobacterium phosphoreum, bioluminescence assay, reverse mutation — + NR Elmore Fitzgerald (1990)... 

The LIA is an immunoassay in which the antigen or antibody are labeled with either a chemiluminescent or bioluminescent tags (41, 58). Luminescent molecules are produced by oxidation reactions. Bis-phenyl oxalates in presence of hydrogen peroxides are used for chemiluminescent assays and luciferin in presence of luciferase enzyme is used for bioluminescent assays. The sensitivity of the LIA s are in the pg/ml or lower range. 

Goicolea A, Barrio RJ, de Balugera ZG, et al. 1998. Study of the toxicity in industrial soils by the bioluminescence assay. J Environ Sci Health Part A 33(5) 863-875. 

Broker, L.E. et al. 2005. Cell death independent of caspases a review. Clin. Cancer Res. 11, 3155-3162. Bulanova, E.G. et al. 1995. Bioluminescent assay for human lymphocyte blast transformation. Immunol. Lett. 46, 153-155. 

Fan, F. and Wood, K. 2007. Bioluminescent assays for high-throughput screening. Assay Drug Dev. Technol. 5 127-136. 

Squatrito, R., Connor, J., and Buller, R. 1995. Comparison of a novel redox dye cell growth assay to the ATP bioluminescence assay. Gynecol. Oncol. 58, 101-105. 

One of the most important quality characteristics of advanced oxidation processes is their ability to reduce the toxicity of an industrial wastewater and to enhance its biodegradability (see Chapter 7.1.5). This criterion can be established by several standardized procedures using different test organisms ranging from microbes to intact animals (cf Tab. 5-1). An easy to perform variant is the bioluminescence assay that uses the inhibition of the bioluminescence intensity of the test organism Vibrio fischeri in the presence of toxic substrates. This is a bioassay used worldwide for the evaluation of toxicity data of individual chemicals or of industrial wastewaters (Froehner et al, 2000, DIN, 1991). Commercial systems are... 

Strehler, Bernard L, Bioluminescence Assay Principles and Practice. 16... 

The results obtained with a protein phosphatase assay and a HPLC/UV/MS method are compared with the results obtained with a bioluminescence assay, which is successfully introduced here for... 

Hsp70 ATPase activity was measured by monitoring the disappearance of ATP substrate over time using a bioluminescence assay as described under Methods. The concentration of the various peptides in the assay mixture was 120 pM. 

Stables, J. Green, A. Marshall, F. et al. A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor. Anal. Biochem. 1997, 252, 115-126. Milligan, G. Rees, S. Chimaeric G alpha proteins their potential use in drug discovery. Trends Pharmacol. Sci. 

Luminescence molecular detectors have also been used for on-line monitoring of dissolution tests and the characterization of toxic residues using bioluminescence assays [28]. 

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