Bioluminescence advantages

Flow cells may also act as reactors. In BL, enzymes may be immobilized inside the cell either by chemical bonding on the inner surface or by entrapping the enzyme as a heterogeneous system by mechanical ways. This approach has the advantage of low consumption of expensive reagents and enhancement of their stability, which is usually low. Many bioluminescent reactions have utilized the benefit of this process. The flow cell is also used as a reactor in the case of electrogenerated chemiluminescence (ECL) when used with FI manifolds. Some of these applications are included in Table 4. 

The advantages of detecting cDNA probes by chemiluminescence and bioluminescence include high sensitivity and simple protocols, using either manual film... 

Directly labeled chemiluminescent systems produce <1 photon/label and require complex chemical synthesis to produce each new labeled molecule (1,2). In contrast, chemiluminescent detection of enzyme labels combines the advantages of a high specific activity label with the convenience of relatively simple coupling chemistries, which use commercial reagents. A number of enzyme labels can be detected via chemiluminescent or bioluminescent reac-... 

Nowadays, Microtox is the most popular bioassay available that uses bioluminescent bacteria as its active element. Analysts find it a useful tool for assessing pollution in different compartments of the environment, possessing as it does both the advantages of bioindicator techniques and the precision of classic instrumental analysis. Here are some examples of its application ... 

A bacterial assay, the Photobacterium test, based on the inhibition of light emission of a bioluminescent bacterium Vibrio fisheri (ISO, 1998), originally developed to test the toxicity of industrial effluents, gives an indication of the effects of the test agent on the oxidative metabolism of the cell. As a bacterial assay its advantages are rapidity and relatively low costs, but naturally the differences between bacterial and mammalian systems make the interpretation of the results even more difficult than with other short-term tests. 

To estimate water quality, bioluminescent biosensors have been devised and successfully used. They are characterized by rapidity and simplicity of use, high sensitivity, and accuracy. The Collection of Luminous Bacteria IBSO (http //www.bdt.org.brA3dt/msdn/ibso) is being used to develop bioassays for monitoring the environment, using lyophilized luminous bacteria and the luminescent system isolated from them. Bioluminescent assays have an advantage over other biological assays luminescence is easy to measure, the method is rapid, and the measurements can be automated. 

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