Biological Methods of Lipid Modification

Biotechnological transformation is powerful tool to effectively utilize a broad variety of plant oils, with the aim to modify their structure for the production of new lipid-based materials with demanded properties and functions. One method of plant oil transformation is based on the direct utilization by microorganisms. Employed oils can be converted to aimed compounds by submerged cultivation or oils, and/or oleaginous plant materials can be utilized during solid state fermentation to useful bioproducts enriched with demanded microbial products. Another biotransformation technique covers the enzymatic modification of oil components to structured lipids with biological properties. 

If the lipids of interest are minor constituents of the sample or present in relatively low concentrations in a complex biological sample, extraction, isolation, and concentration steps usually precede TLC. Numerous sample preparation methods are available, and personal choice often dictates which one will be used. Of all the procedures, that of Folch et al. (70), ora modification of the original procedure, is used more frequently than any other. Table 4 lists numerous procedures used for the sample preparation of lipids. 

Standard Method 5520E Sludge Suitable for biological lipids and mineral hydrocarbons. Not suitable for low-boiUng fractions. Method E is a modification of Method D. 

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