Biological cells and tissues

TOF-SIMS has important potentials in many areas of life science, in fundamental and applied research as well as in product development and control. This holds for the characterization of biological cells and tissues, of sensor and microplate arrays, of drug delivery systems, of implants, etc. In all these areas, relevant surfaces feature a very complex composition and structure, requiring the parallel detect ion of many different molecular species as well as metal and other elements, with high sensitivity and spatial resolution requirements, which are exactly met by TOF-SIMS. 

Electron microscopic techniques can be used in order to visualize the architecture of the network. We have adapted (P. Favard, N. Favard, M. Djabourov, J. Leblond, to be published) a method recently proposed by Heuser (9) (1978), for the observation of biological cells and tissues. ... 

Bereiter-Hahn, J. (1995). Probing biological cells and tissues with acoustic microscopy. In Advances in acoustic microscopy, Vol. 1 (ed. G. A. D. Briggs), pp. 79-115. Plenum Press, New York. [197]... 

Electrophysiology — Electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in animals, plants, bacteria, and insects. It involves measurements of electrical -> potentials or - current on a wide variety of scales from single ion channel, to whole tissues like the heart, muscles, phloem,... 

For a particle diameter of about 1 fim, x 2 x l(F cm-, and g a 0.2, a Asg of ca. 3SOOO is obtained at room temperature. The dispersion occurs in the low-frequency range of 1—10 kHz, in quantitative agreement with the relaxation time given by (46). There can be little doubt that the still more remarkable low-frequency dielectric dispersion of biological cells and tissue is also due to such counterion-polarization effects. 

HI. Haggart, R., and Leaback, D. H., Determination of quantitative, luminogenic images of macromolecule components of biological cells and tissues. Anal Chim. Acta 227, 257-265... 

The dielectric properties of biological cells and tissues have been of interest for nearly a hundred years. The early work of Maxwell [1] and Wagner [2], Fricke [3], Cole [4], and... 

Metal Composition in Biological Cells and Tissues, Anal. Bioanal. Chem.,... 

N.W., and Gardner, P. (2012) FTIR microscopy of biological cells and tissue data analysis using resonant Mie scattering (RMieS) EMSC algorithm. Analyst, 137, 1370-1377. 

In this review, we focus on the use of plant tissue culture to produce foreign proteins that have direct commercial or medical applications. The development of large-scale plant tissue culture systems for the production of biopharmaceutical proteins requires efficient, high-level expression of stable, biologically active products. To minimize the cost of protein recovery and purification, it is preferable that the expression system releases the product in a form that can be harvested from the culture medium. In addition, the relevant bioprocessing issues associated with bioreactor culture of plant cells and tissues must be addressed. 

Another free radical, which is supposedly formed in biological systems is the nitric dioxide N02. This radical is much more reactive than nitric oxide its rate constants with thiols, urate, and Trolox C are about 107—10s lmol-1 s 1 [88,89] (Table 21.1). It has been proposed [88] that thiols are dominant acceptors of N02 in cells and tissues while urate is a major scavenger... 

It has been proposed [91] that nitric dioxide radical formation during the oxidation of nitrite by HRP or lactoperoxidase (LPO) can contribute to tyrosine nitration and be involved in cell and tissue injuries. This proposal was supported in the later work [92] where it has been shown that N02 formed in peroxide-catalyzed reactions is able to enter cells and induce tyrosyl nitration. Reszka et al. [93] demonstrated that N02 mediated the oxidation of biological electron donors and antioxidants (NADH, NADPH, cysteine, glutathione, ascorbate, and Trolox C) catalyzed by lactoperoxidase in the presence of nitrite. 

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