Biolistic particle delivery

Expression of GUS constructs with various seed specific promoters, including lectin (4) were examined by means of bombardment with DNA-coated-gold particles. 1 jiig Plasmid DNA was coated onto 1 mg gold particles and delivered onto the surface of embryo tissues by a He-driven Biolistic Particle Delivery System (Bio-rad, Model PDS-1000). Transient expression of GUS was assessed by histochemical staining according to Jefferson et al. (5). 

Deliver the plasmid-coated gold or tungsten particles into the detached leaves using a particle delivery system of choice (e.g., Bio-Rad Biolistic PDS-1000 HE particle delivery system) following the manufacture s protocol. 

This technique, also referred to as biolistics (biology + ballistics), is a direct DNA delivery method developed by Sandford and co-workers [16-18]. This technique employs microprojectiles or high velocity particles coated with DNA to deliver exogenous genetic material into the target cell or tissue, which is subsequently in vitro cultured, and regenerated to produce mature transformed plants. 

Shock Tube as Vaccine Delivery System A unique form of powder delivery system, a biolistic system, has been developed [19]. This novel technology accelerates microparticles by a gas flow behind a traveling shockwave, so that they can attain sufficient momentum to penetrate the skin and thus achieve a pharmacological effect. One of the most recent developments is a mouse biolistic system, used in immunological studies. These studies require powdered vaccine to be delivered into the epidermis of the mouse with a narrow and highly controllable velocity distribution and a uniform spatial distribution. The preliminary results demonstrate the overall capability of a newly designed supersonic nozzle to deliver the particles to the skin targets with a more uniform velocity and spatial distribution. CTD has been utihzed to characterize the complete operation of a prototype mouse biolistic system. 

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