Biocatalysis development

Bergeron S, Chaplin D, Edwards JH, Ellis BS, Hill CL, Holt-Tiffin K, Knight JR, Mahoney T, Osborne AP, Ruecroft G (2006) Nitrilase-catalyzed desym-metrization of 3-hydroxyglutaronitrile preparation of a statin side-chain intermediate. Org Proc Res Dev 10 661-665 Burns M, Weaver J, Wong J (2005) Stereoselective enzymic bioconversion of aliphatic dinitriles into cyano carboxylic acids. WO 2005100580 DeSantis G, Zhu Z, Greenberg W, Wong K, Chaplin J, Hanson SR, Farwell B, Nicholson LW, Rand CL, Weiner DP, Robertson D, Burk MJ (2002) An enzyme library approach to biocatalysis development of nitrilases for enantioselective production of carboxylic acid derivatives. J Am Chem Soc 124 9024-9025... 

J. 2002. An enzyme library approach to biocatalysis development of nitrilases for enantioselective production of carboxylic acid derivatives./. Am. Chem. Soc.,124(31), 9024-9025. 

CaUeri, E. Temporini, C. Furlanetto, S. Loiodice, R 45. FracchioUa, G. Massolim, G. Lipases for biocatalysis Development of a chromatographic hioreactor. J. Pharm. 

Previously, we have shown that functional secretion of OPH molecules into the periplasmic space induced about 2.8-fold higher specific whole cell OPH activity [10]. From the detail reaction kinetic studies in this work, we showed that this periplasmic space-secretion strategy provided much improved bioconversion capability and efficiency ( 1.8-fold) for Paraoxon as a model organophosphate compound. From these results, we confirmed that Tat-driven periplasmic secretion of OPH can be successfully employed to develop a whole cell biocatalysis system with notable enhanced bioconversion efficiency and capability for environmental toxic organophosphates. 

The use of ionic liquids (ILs) to replace organic or aqueous solvents in biocatalysis processes has recently gained much attention and great progress has been accomplished in this area lipase-catalyzed reactions in an IL solvent system have now been established and several examples of biotransformation in this novel reaction medium have also been reported. Recent developments in the application of ILs as solvents in enzymatic reactions are reviewed. 

Important topics in biocatalysis for organic synthesis are described in this book, for experts and non-experts. Especially, the book focuses on those reactions that are under development now and will be more significant in the future. Therefore, each chapter describing a specific theme summarizes not only the present state but also the direction of the research. The prospects and dreams that will become possible, using biocatalysis, in the future to construct a sustainable society are also included. 

In a biphasic medium, two situations are distinguished for the reaction. Biocatalysis occurs at the liquid-liquid interface [42,43] or in the bulk of the aqueous phase [25,27]. Models have been developed for both types, and interaction between mass transfer and enzyme-catalyzed reactions has been also studied. 

BIOCATALYSIS FOR THE PHARMACEUTICAL INDUSTRY Discovery, Development, and Manufacturing... 

Biocatalysis for the pharmaceutical industry discovery, development, and manufacturing/Junhua Tao, Guo-Qiang Lin, Andreas Liese. p. cm. 

Consequently, many successful stories and a number of reviews have been reported recently in developing biocatalysis for the pharmaceutical industry, across drug discovery, development, and manufacturing. The book is dedicated to these advances, and divided into four parts ... 

Chapter 14 discusses the recent impact of biocatalysis in green chemistry and chemical development. 

Ran, N., Zhao, T., Chen, Z. and Tao, J. (2008) Recent applications of biocatalysis in developing green chemistry for chemical synthesis at industrial scale. Green Chemistry, 10, 361-372. 

The complexity of today s pharmaceutical compounds and an increasing awareness of the environmental impact of traditional chemical syntheses have opened the door to biocatalysis. Directed evolution is an integral tool in the development of synthetic enzymes, ensuring they are suitable for use in an industrial setting. The past success of this approach indicates that it will continue to provide many examples of safe and efficient production of chemical intermediates and medical compounds. 

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