Binding relationships

The same set of measurements for DNP-Leu as selectand and 0-9-allylcarbamoyl-10,11-dihydroquinidine as selector displayed a considerably different picture. While no indication for H-bond formation between the aromatic a-amino group and the carbamate of the selector could be deduced, the IR spectra of free and respective complexed forms suggested the occurrence of K-K-interactions of the DNP group as evidenced by a shifted C=C stretching vibration from 1594 to 1588 cm in the stronger bound 5-complex (force constant weakened due to delocalized electrons). This type of shift was not found in the weak TJ-complex, but in the corresponding 5-complex of DNP-N-methyl-Leu as well. The spectral data nicely reflect the binding relationships in HPLC where both DNP-Leu and DNP-N-methyl-Leu are well separated with comparable separation factors, but with an elution order that is opposite compared to DNB-Leu. 

These equilibrium-binding relationships give rise to four different kinetic responses competitive inhibition, uncompetitive inhibition, non-competitive inhibition, mixed inhibition. Details of the kinetics of these types of inhibition and how dissociation constants for the reactions can be measured are provided in Appendix 3.6. 

According to the kind of interaction forces and binding relationship between the solid and the adsorbed particle, we distinguish the physical adsorption and chemisorption. The real state of most systems is between these two extremes. For a detailed study of both adsorption mechanisms see e.g. Fripat et al.80. ... 

Figure 6. Structure-binding relationships of various antisera for cannabinoids...

For the following applications, additive quantities are needed that can reproduce the inter-molecular binding relationships, e.g. partition coefficients are based on such relationships. 

Timofei, S., Kurunczi, L., Schmidt, W. and Simon, Z. (1995) Structure-affinity binding relationships of... 

T. Hogberg, A physicogenetic method to assign ligand-binding relationships between 7TM receptors, Bioorg. Med. Chem. Lett. 2005, 15, 3707-3712. 

The conservation and flushing effects observed in vitamin B6 metabolism can be simulated by binding relationships. 

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