Barley washing

One practical aspect of the procedure for monitoring carbon flow following C labeling is the need to separate roots from the soil for analysis. Incomplete removal of roots can lead to an overestimation of rhizodeposition, but overzealous washing of soil may lead to leaching of " C or loss of fine roots. This problem has been examined in detail for wheat and barley, and procedures to correct for these errors have been developed (69). 

J. Swinnen, J. A. van Veen, and R. Merckx, Losses of C from roots of pulse-labelled wheat and barley during washing from soil. Plant Soil /66 93 (1994). 

Proteolytic enzymes Animals, including insects and other arthropods or their larval forms Dusts from barley, oats, rye, wheat or maize, or Biological washing powders and the baking, brewing, fish, silk and leather industries Research and educational laboratories, pest control and fruit cultivation The baking or flour milling industry or on farms... 

Glucan in dry pasta products can be determined using the Basic Protocol. Since pasta particles tend to be firmer than other cereal products, cooking time may be extended if necessary (up to 15 min see Basic Protocol, step 6). Fresh pasta products require additional sample preparation steps. It can be analyzed in either pureed or dried form. In ready-to-eat meals, P-glucan may be incorporated into a side dish such as pasta or pot barley. Because it will most likely be mixed with a sauce, which will interfere with P-glucan determination, the pasta must be washed prior to analysis. 

Moilanen and his co-workersalso obtain increasing reactivity profiles with conversion, except for peat. They expect such increasing reactivity because of pore development structure, enhanced by the catalytic effect of the ash, since the ratio catalyst/carbon increases with char conversion. Stoltze et al. obtain similar profiles with barley straw. Rensfelt et al. find as well increasing reactivity with conversion, and a characteristic shape of the reactivity profile for each fuel, having each fuel the same curve independent of temperature. However, for washed barley chars, Sorensen et al. find a decreasing reactivity as a function of conversion. 

Having first washed the barley in cold water, boil it for a short time in about half a pint of water throw away this water then pour upon the barley five pints of boiling water boil it next until half the quantity of the water be evaporated, and afterwards strain it. 

Geneva is made from a mixed wash of malted barley, rj-e, and maize in equal proportions, the fermented wash being distilled in a pot still and subsequently flavoured. 

Barley Water. Wash away with cold water all extraneous matter from 5 ounces pearl barley then boil for a short time in i pint water, throw this away, and boil the parboiled barley in 4 pints water down to 2 pints, and strain. 

Nilan, R. A. (1964), The cytology and genetics of barley. Res. Studies State Univ. Wash. 32 (1), Monographic Suppl. No. 3. 

FIGURE 1. Barley thylakoids (T) were incubated with labeled pLHCP.j ollowing incubation of 30 minutes at 25C, the thylakoids were fractionated into grana (G)- and stroma (S)-lamellae, or left intact. The three membranal fractions were then washed with either 2M NaBr (1), or buffer (2). Equal amounts of membranes from each fraction (10 ug Chi) -- and the translation products (tp) were... 

Briggs, D. (2004). The benefits of washing barley with hot water a preliminary study. Technical Quarterly Master Brewers Association of Americas, 4, 390-393. 

Fig. 4. Immunoprecipitation of AB A-induced proteins in barley aleurone layers with antibodies against barley lectin and a-amylase inhibitor. Aleurone layers were incubated with or without 2 X 10 M ABA for 24 h. Labeling with 50 jaCi/ml p S] methionine took place in the final h of incubation. In A proteins were extracted with 0.1 M Na acetate (pH 5.5) in B proteins were extracted with 2% Triton X-100 at 40 C for 30 min. Aliquots of the extracted proteins were reacted with the antibodies and then precipitated with Staphylococcus aureus Cowan strain 1. The pellets were washed and analyzed by SDS-PAGE. Fluo-rograms of the gels are shown. Mol wt marker are indicated by bars at left. L.-S. Lin and T.H.D. Ho, unpublished...

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