Tests bacterial inhibiting

Adsorption and the inoculated material are two factors which have a great 

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The calf antibiotic and sulfa test (CAST), the fast antimicrobial screen test (FAST), and the swab test on premises (STOP) are all bacterial inhibition tests developed at the US Department of Agriculture Food Safety and Inspection Services to screen kidneys for antimicrobial drug residues at slaughter (97-101). For CAST and FAST, an incision is made with a knife, whereas for STOP the tissue is macerated with the shaft of the swab. For all three tests, cotton swabs are inserted into kidney tissue to soak up fluids. Swabs are then incubated on inoculated medium with a disc containing an antibiotic standard used to monitor the viability of the organism and its growth inhibition. 

Korsrnd GO, Boison JO, Nonws JE, MacNeU JD. Bacterial inhibition tests nsed to screen for antimicrobial veterinary drng residnes in slanghtered animals, J. AOAC Int. 1998 81 21-24. 

The Guthrie test is a bacterial inhibition assay for the measurement of phenylalanine. 

The first steps in bypassing of the biological, technological, and financial burden of live stock culturing or maintenance were made more than 20 years ago through the development of a bacterial luminescence inhibition test [34,35] this bioassay is presently known and used worldwide as the Microtox test. The revolutionary principle of this test is that it uses a lyophihzed strain of a (marine) bacterium Photobacterium phosphoreum). This makes the bioassay apphcable anytime, anywhere, without the need for continuous culturing of the test species. 

Vibrio fischeri, bacterial luminescence inhibition test or Microtox assay (DEVL34, 1998). 

Bacterial strains tested Diameter of the inhibition zone in mm... 

An example of the use of a highly specialised cell type to study targeted toxic effects on the cellular metabolism is the recently developed boar spermatozoon motility inhibition test (Andersson et al., 1998). The motility of a spermatozoon depends on the integrity of mitochondrial functions, and thus the action of toxins affecting the energy metabolism is very rapidly detected as reduction of motility. Other end points that can be measured are plasma membrane integrity, astrodome function, and total cellular ATP and NAD reduction. This test has been particularly useful in the detection of certain types of bacterial toxins from various enviromnental and food sources. 

The use of photosynthetic enzymes isolated from plants has been implemented in a toxicity monitor (LuminoTox, Lab Bell Inc., Shawinigan, Canada). This system can detect a range of compounds such as hydrocarbons, herbicides, phenols, polycyclic aromatic hydrocarbons (PAHs), and aromatic hydrocarbons. These enzymes have been coupled to screen-printed electrode and have been demonstrated to be able to detect triazine and phenylurea herbicides [79]. Other enzyme inhibitions have been used to detect biotoxins from plant, animals, bacterial, algae, and fungal species (e.g., ricin, botulinum toxins, mycotoxins, cyanobacterial toxins). However, since the identity and specificity of the above toxic compound can be very important during the analysis, other sensor systems such as immunosensors may be preferred to give a better indication to toxin type and identity than the use of enzyme inhibition tests. 

Fig. 9 Bacterial inhibition of forming film toward Bacillus subtilis (left) and Escherichia coli (right) through the agar diffusion test...

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