Minimal medium bacteria

Figure 3 Root fingerprints of Pseudomimets sp. associated with barley seedlings showing the production of siderophore by actively growing bacteria located in the zone of elongation behind the root tips. Root.s were pressed on to an iron-deficient minimal medium selective for Pseudomonas. After growth of the colonies, the production of siderophore was visualized by exposure of the agar plate to ultraviolet light, which causes the siderophore to Huoresce.

Therkddsen, M., Isaksen, M. F., and Lonstein, B. A. (1997). Urea production by the marine bacteria Delaya venusta and Pseudomonas stutzeri grown in a minimal medium. Aquat. Microb. Ecol. 13, 213-217. 

The misincorporation of norleucine for methionine was known to occur in bacteria when high level synthesis of recombinant proteins were induced in minimal medium fermentation (1,2). This misincorporation was detected in the production of N-labeled recombinant human leptin produced using minimal medium conditions, however, is not present in the clinical samples produced using other fermentation conditions. The mechanism for the misincorporation was believed to involve the de novo synthesized norleucine which bypasses the leucine biosynthetic pathway and enters directly into the... 

In B. subtilis, the pathway from chorismate to tryptophan is feedback-inhibited by tryptophan, which suppresses anthranilate synthase activity. Mutant B. subtilis that lacks tryptophan synthetase can grow on minimal medium only when supplemented with exogenous tryptophan. Under these conditions, none of the intermediates in the tryptophan biosynthetic pathway from anthranilate to indole 3-glycerol phosphate are produced. However, when the bacteria have depleted the medium of tryptophan, the levels of those intermediates increase, even though there is no net production of tryptophan. Why ... 

The Ames test is extranely simple and fast. A strain of bacteria is cultured that is unable to grow on a minimal medium devoid of histidine. When mixed with the suspected mutagen, some of the bacteria may mutate into a bacterial strain capable of growth on the minimal growth medium. Presence of bacterial colonies growing on plates containing the minimal medium indicates that the substance is, indeed, capable of mutagenicity. 

Ln vitro studies on HT-29 epithelial cells were made in order to test the capacity of L. johnsonii strains to decrease S. enterica serovar typhimurium UKl-lux invasion (Muller et al., 2011). It was shown that the L. johnsonii proved its exclusion properties just when the probiotic bacteria are growing in minimal medium supplemented with fatty acids. In these conditions, L. johnsonii reduced significantly Salmonella spp. adhesion and invasion. If the L. johnsonii was grown in a medium supplemented with linoleic and linolenic acids, its adhesion was also possible even when the HT-29 cells were infected with Salmonella spp. (Muller et al., 2011). The mechanism proposed in order to explain how probiotics prevent pathogens from infecting cells is that they sterically hinder the adherence of pathogens (Foumiat et al., 1992). 

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