Bacillus brevis

Chemically synthesised D,L-hydantoins prepared from the corresponding aldehydes via die Bucherer Berg reaction are converted by the bacterial cells (Bacillus brevis), containing a D-spedfic hydantoinase, to a mixture of D-N-carbamoyl amino acid and L-hydantoin. The latter compound undergoes rapid and spontaneous racemisation under the conditions of the reaction, therefore, in principle 100% of the hydantoin is converted into the D-N-carbamoyl compound. The D-amino add is obtained after treatment of the D-N-carbamoyl compound with nitrous add. This process is operated on an industrial scale by the Japanese firm Kanegafuchi. 

Bacillus brevis) Bacillus HM105 olpA 874/29 U38842... 

Que L, J Widom, RL Crawford (1981) 3,4-Dihydroxyphenylacetate 2,3-dioxygenase a manganese(II) dioxygenase from Bacillus brevis. J Biol Chem 256 10941-10944. 

Naturally occurring gramicidin is a mixture of at least six linear pentadecapeptide antibiotics of closely related structure produced by Bacillus brevis 207). The amino acid sequence of the major component [Valjgramicidin A was reported by Sarges and Witkop208 in 1965 ... 

The Gram-positive bacteria Bacillus subtilis, Bacillus megaterium and Bacillus brevis are also used for heterologous expression. Terpe [13] presented a table with some pharmaceutically and industrially relevant proteins expressed with this host. It is the most used prokaryotic host after... 

Kajino, T., Saito, Y., Asami, O. etal. (1997) Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system. Journal of Industrial Microbiology Biotechnology, 19 (4), 227-231. 

D-4-Hydroxyphenyl glycine 9 Ampicillin/ amoxycillin Antibacterial Hydrolysis Bacillus brevis Immobilized cells [8]... 

Tyrothricin was obtained by acidification of the fermentation broth of Bacillus brevis to precipitate the antibiotic activity along with various proteins and then dissolving the antibiotic complex in alcohol. The alcohol was removed under vacuum, the residue was washed with ether, then redissolved in alcohol and finally reprecipitated with 1% sodium chloride5 6. 

Stokes and Woodward established that Bacillus brevis produces the antibiotic in stationary cultures of both complex natural and synthetic media90. They noted that production of the antibiotic occurred in an aerated synthetic medium but not in an aerated complex nitrogenous medium. 

Akers, Lee and Lipmann have isolated two enzymes from Bacillus brevis that are responsible for the synthesis of the initial portion of the gramicidins223. 

CASRN 50471-44-8 molecular formula C12H9CI2NO3 FW 286.11 Biological Golovleva et al. (1991) studied the aerobic biodegradation of vinclozolin in the laboratory and under field conditions using enrichment cultures. Four of the 36 microbial strains isolated were the most active. These were Bacillus cereus 625/1, Bacillus brevis 625/2, Pseudomonas Huorescens 10/3, and 8/28. Under aerobic conditions. Bacillus cereus 625/1 and Pseudomonas Huorescens 8/28 utilized 87-90% vinclozolin as the sole carbon and energy source. Degradation products identified were 2((((3,5-dichlorophenyl)carbamoyl)oxy)2-methyl)-3-bute-... 

In 1939, Rene Dubos, Waksman s former postdoctoral student, extracted two chemicals, tyrocidine and gramicidin, from the soil germ Bacillus brevis. These chemicals cured bacterial infections in cattle but were too toxic for humans. This discovery prompted a number of scientists to expand the search for microbes in the soil, microbes capable of making chemicals that could kill disease-causing bacteria in humans. 

Hitchcock, M.J.M. Hodgson, B. Lysine- and lysine-plus-threonine-inhibi-table aspartokinases in Bacillus brevis. Biochim. Biophys. Acta, 445, 350-363 (1976)... 

Louw, M.E. Reid, S.J. James, D.M. Watson, T.G. Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus brevis. Appl. Microbiol. Biotechnol., 42, 78-84 (1994)... 

Gramicidin A (a pentadecapeptite from Bacillus brevis) [11029-61-1]. Purified by countercurrent... 

Gramicidin C (a pentadecapeptite from Bacillus brevis) [9062-61-7]. Same as Gramicidin A since they are isolated together and separated. [Sarges and Witkop Biochemistry 4 2491 1965 as well as references above for Gramicidin A]. 

Gramicidin A (a pentadecapeptite from Bacillus brevis) [11029-61-1]. Purified by countercurrent distribution from C6H6-CHCI3, MeOH-H20 (15 15 23 7) with 5000 tubes. Fractions were examined by UV (280nm) of small aliquots. Separation from Gramicidin C and other material occurred after 999 transfers. [Gross and Witkop Biochemistry 4 2495 1965] Bauer et al. Biochemistry 11 3266 1 972]. Purified finally by... 

Structure of a Peptide Antibiotic from Bacillus brevis Extracts from the bacterium Bacillus brevis contain a peptide with antibiotic properties. This peptide forms complexes with metal ions and apparently disrupts ion transport across the cell membranes of other bacterial species, killing them. The structure of the peptide has been determined from the following observations. 

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