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Assays high-content/phenotypic

Smaller compound sets and focused compound libraries can be used to screen more broadly for physiological phenotypes. In the typical high-content screening experiment, the effects of compounds on cellular assays are captured... [Pg.6]

High-content screening (HCS) is a cell assay method in which phenotypic changes in live cells... [Pg.309]

In this chapter, we describe a protocol for the systematic perturbation of patient-derived cell lines using small-molecule probes, which is both scalable to high-throughput workflow and generalizable to a variety of assays. In the protocol presented here, multiple patient-derived lymphoblastoid cell lines either mutant or wild type at a defined genetic locus (e.g., HNF4a) are perturbed by an annotated chemical library. After sufficient incubation, cells are subjected to a phenotypic assay, in this case a luminescence-based readout of cellular ATP content, that aims to quantify the effect of compounds on oxidative phosphorylation, viability, or other relevant traits. The effect of each compound is expressed as a metric that reflects the difference in compound-induced phenotypes between mutant and wild-type cells. These ratios are then... [Pg.22]

See other pages where Assays high-content/phenotypic is mentioned: [Pg.271]    [Pg.54]    [Pg.102]    [Pg.491]    [Pg.494]    [Pg.729]    [Pg.404]    [Pg.405]    [Pg.406]    [Pg.59]    [Pg.154]    [Pg.189]    [Pg.256]    [Pg.257]    [Pg.259]    [Pg.259]    [Pg.249]    [Pg.1724]    [Pg.194]    [Pg.2]    [Pg.65]    [Pg.174]    [Pg.750]    [Pg.753]    [Pg.201]    [Pg.204]    [Pg.97]    [Pg.424]    [Pg.45]    [Pg.67]    [Pg.68]    [Pg.293]    [Pg.297]    [Pg.318]    [Pg.377]    [Pg.326]    [Pg.27]    [Pg.43]    [Pg.167]    [Pg.37]    [Pg.313]    [Pg.645]    [Pg.21]   
See also in sourсe #XX -- [ Pg.494 ]



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High assay

High content

Phenotype

Phenotype/phenotyping

Phenotypic

Phenotypic assays

Phenotyping

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