Arthrobacter simplex, immobilization

TG Park, AS Hoffman. Immobilization of Arthrobacter simplex in thermally reversible hydrogels Effect of gel hydrophobicity on steroid conversion. Biotech Prog 7 383-390, 1991. 

The present type of thermosensitive gel has been applied in the immobilization of Arthrobacter simplex cells, and the bioconversion of a steroid (hydrocortisone -> prednisolone by 3-ketosteroid-A1-dehydrogenase in A. simplex) was examined using the thermal cycling method [65]. In this case, the thermal cycling was... 

The bioconversion of hydrocortisone to prednisolone by free, immobilized, and reused immobilized cells of Bacillus sphaericus ATCC 13805, B. sphaericus SRP III, and Arthrobacter simplex 6946 was investigated in an aqueous as well as a two-liquid-phase system using various organic solvents [141]. The immobilized cells... 

Immobilized micTOorganisms, in particular Arthrobacter simplex and Nocardia rhodocrous, and fungal spores, in particular those from Septomyxa t finis, can also be used to effect dehydrogenations. Dehydrogenations may also be advantageously carried out in the presence of hydrocarbon solvents, for example the conversion of ba-methylhydrocortisone (62) to 6a-methylpredisolone (63 equation 20) with Arthrobacter simplex. ... 

Arthrobacter simplex cells coprecipitated with calcium alginate gel have been used for the production of prednisolone from cortisol.The conversion of hydrocortisone into prednisolone has been czxned out ismg Arthrobacter simplex cells entrapped in a polypropylene glycol-polyethyleneglycol copolymer,in a maleic polybutadiene gel, or in urethane prepolymers.Mass-transfer effects on the rate of isomerization of D-glucose into D-fructose catalysed by commercially available immobilized Arthrobacter cells have been investigated and a theoretical treatment proposed. 

Achromobacter aceris cells have been immobilized by entrapment in polyacrylamide and applied to the continuous production of NADP. Cells of Arthrobacter simplex entrapped in polyacrylamide show steroid-A -dehydro-genase activity and catalyse the transformation of cortisol to prednisolone. Use of the immobilized cells in semicontinuous batchwise operations was investigated using various nutrient media. 

Park TG, Hoffman AS. Immobilization of Arthrobacter simplex in a thermally reversible hydrogel Effect of temperature cycling on steroid conversion. Biotechnol Bioeng 1990 35 152-159. 

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See also in sourсe #XX -- [ ]

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