Antigens, immunochemical studies

Chapters 17 through 21 deal with carbohydrate-enzyme systems. Hehre presents some new ideas on the action of amylases. Kabat presents some new immunochemical studies on the carbohydrate moiety of certain water-soluble blood-group substances and their precursor antigens. Hassid reviews the role of sugar phosphates in the biosynthesis of complex saccharides. Pazur and co-workers present information obtained by isotopic techniques on the nature of enzyme-substrate complexes in the hydrolysis of polysaccharides. Gabriel presents a common mechanism for the production of 6-deoxyhexoses. An intermediate nucleoside-5 -(6-deoxyhexose-4-ulose pyrophosphate) is formed in each of the syntheses. 

Immunochemical Studies on the Carbohydrate Moiety of Water Soluble Blood Group A, B, H, Lea, and Leb Substances and Their Precursor I Antigens... 

Figure 2. Immunochemical studies ofthe specificity ofrabbit antibodies elicited against Parp(al—3)D-Manp-BSA glycoconjugates (02). Top structures of the Salmonella O-antigen-specific saccharides used. Center Inhibition of precipitation in mixtures of anti-PM-BSA serum andS. paratyphi A PS. Key Center V, Octasaccharide S. paratyphi A. var. Durazzo ft.. Dodecasaccharide S. paratyphi A. var. Durazzo (II) , Parp D-Manp 4 OMe a., Parp -jpi- D-Manp O-0-NHCSNH(CH2)5COOH (VIII) m. Parp -j-i- D-Manp O-0-NHCOCF. (VII) U, Parp OMe A, Dodecasaccharide...

G. Schiffman, E. A. Kabat, and W. Thompson, Immunochemical studies on blood groups. XXXn. Immunochemical properties of and possible partial structures for the blood group A, B, and H antigenic determinants, Biochem. J., 3 (1964) 587-593. 

The concept of receptors is credited to the independent work of Paul Ehrlich (1845-1915) and J. N. Langley (1852-1926). With Ehrlich, the concept appeared to originate from his immunochemical studies on antibody-antigen interactions. Based on the high degree of specificity of antibodies for antigens, Ehrlich postulated the existence of stereospecific, complementary sites on the two molecules. Similar... 

Protein A binds to the F,. region of IgG and does not interfere with the antigen binding capacity of IgG, thus FITC-Protein A can form tertiary complexes with antigen and antibody (IgG) . FITC-labeled protein A has been applied in different immunochemical studies and separations of cells bearing IgG or other... 

On numerous occasions in immunochemical study the need arises to link proteins to particles, to polymerize proteins or to form covalent conjugates of proteins and smaUer peptides. The availability of reagents that are simple and effective while largely preserving the native antigenicity of... 

Used in immunochemical studies of antigenic determinants of fibrinogen and fragments... 

L2. Lapresle, C., Kaminski, M., and Tanner, C. E., Immunochemical study of the en-Z3unatic degradation of human serum albumin. An analysis of the antigenic structure of a protein molecule. J. Immunol. 82, 94-102 (1959). 

Wood C, Rabat EA. Immunochemical studies of conjugates of isomaltosyl oligosaccharides to lipid. I. Antigenicity of the glycohpids and the production of specific antibodies in rabbits. J Exp Med 1981 154 432-449. 

Basing on the above findings provided by the workers above mentioned, we started a series of immunochemical studies of yeast mannans. In 1968, we reported analysis of the antigenic determinant groups of the mannan of S. cerevisiae, in which the mannan... 

Schlossman SF, Levine H (1967) Immunochemical studies on delayed and arthus-type hypersensitivity reactions. I. The relationship between antigenic determinant size and antibody combining site size. J Immunol 98 211-219 Schlossman SF, Yaron A, Ben-Efraim S, Sober HA (1965) Immunogenicity of a series of, N-DNP-L-lysines. Biochemistry 4 1638-1645 Schlossmann SF, Levine H, Yarou A (1968) Studies on the specificity of antibody to 2,4-dinitrophenyl-poly-L-lysines. Biochemistry 7 1-7 Schneider CH, de Week AL (1967) The reaction of benzylpenicillin with carbohydrates at neutral pH with a note on the immunogenicity of hapten polysaccharide conjugates. Im-munochemistry 4 331-343... 

Taketomi T, Yamakawa T (1963) Immunochemical studies of lipids. I. Preparation and immunological properties of synthetic psychosine-protein antigens. J Biochem (Tokyo) 54 444-451... 

Murakami, W. T., Van Vunakis, H., Grossman, L., Levine, L. Immunochemical studies of bacteriophage deoxyribonucleic acid. II - Characterization of the active antigen. Virology 14, 190-197 (196I). 

A complete and valid comparative immunochemical analysis of lipopoly-saccharides of a genus or species can be carried out only if a serological classification of the respective genus is available. Such a classification exists [13] for Salmonella, E. coli, Shigella, and recently for Citrobacter [36, 88], and immunochemical studies on their O antigens have been performed in the last decades [74]. 

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