Antibody analysis

Murphy, R.E., Callans, S., Gonzales, M., Huyghe, B. (2005). Two-dimensional liquid chromatography for intact antibody analysis in serum. Presented at the International Symposium on the Separation of Proteins, Peptides and Polynucleotides. (ISPPP 2007) St. Pete Beach, FL. 

Mezzanzanica, D., S. Canevari, S. Menard, S.M. Pnpa, E. Tagliabue, A. Lanzavecchia, and M.I. Colnaghi, Human ovarian carcinoma lysis by cytotoxic T cells targeted by bispecific monoclonal antibodies analysis of the antibody components. Int J Cancer, 1988. 41(4) 609-15. 

Shams ili S, Grefkens J, de Leeuw B, van den Bent M, Hooijkaas H, van der Holt B, et al. Paraneoplastic cerebellar degeneration associated with antineuronal antibodies Analysis of 50 patients. Brain 2003 126(Pt. 6) 1409-1418. 

TK/antibody analysis Maternal milk/blood, infant blood... 

We know of no specific review articles that discuss only affinity CE, though there is a very recent review that describes the use of CE for antibody analysis, often with applications that utilize antibody-antigen recognition called immunoaffinity or affinity CE, as above (40). At times, recent texts also contain sections that discuss affinity recognition in CE, such as that by Righetti (10). Because most applications of CE for combinatorial library searching... 

Finally, financial support and technical collaborations have been provided NU in antibody areas by Pharmacia and Upjohn Pharmaceutical Company, through the Animal Health and Drug Metabolism Division (J. Nappier and G. Fate). Additional antibody analysis collaborations have been possible through SmithKline Beecham Pharmaceuticals (D. Nesta and J. Baldoni). These contracts have allowed us to become involved in affinity and immunoaffinity CE areas. Isco Corporation, Thermo Separation Products (Thermo Quest), and Waters Corporation have all donated major instrumentation, materials, and supplies to our efforts in the areas of affinity CE. Colleagues at Supelco, Phase Separations, Ltd., J W Scientific, and Unimicro Technologies have all donated coated or packed capillaries for studies in CE and CEC. We are very appreciative of all these collaborations and technical/financial assistance in developing CE, CIEF, and, most recently, ACE approaches for proteins and antibodies. 

Zucker, D. R. and Murphy, J. R. (1984) Monoclonal antibody analysis of diphtheria toxin—I. Localization of epitopes and neutralization of cytotoxicity. Mol. Immunol. 21(9), 785-793. 

Zimma, A. M., Rosen, S. T., Spies, S. M Golman-Leilin, R Kazikiewicz, J. M., Silverstein, E. A., and Kaplan, E. H. (1988) Radioimmunotherapy of patients with cutaneous T-cell lymphoma using an iodine-131-labeled monoclonal antibody analysis of retreatment following plasmapheresis../. Nucl. Med. 19,174-180. 

Franko MC, Gibbs CJ, Rhoades DA, Gajdusek DC (1987) Monoclonal antibody analysis of keratin expression in the central nervous system. Proc. Natl Acad. Scl USA, 84, 3482-3485. 

Ives, T. J., Manzewitsch, P., Regnery, R. L., Butts, J. D., and Kebede, M. (1997). In vitro susceptibilities of Bartonella henselae, B. quintana, B. elizabethae, Rickettsia rickettsii, R. conorii, R. akari, and R. prowazekii to macrolide antibiotics as determined by immunofluorescent-antibody analysis of infected Vero cell monolayers. Antimicrob. Agents Chemother. 41,578-582. 

In a study of antibody analysis by SEC, it was observed that the optimum pH and ionic strength for good peak shape and noninteraction differed between antibodies [40]. This again emphasized the necessity of mobile phase optimization for attainment of ideal SEC. 

Arahata, K, Engel AG. (1984) Monoclonal antibody analysis of mononuclear cells in myopathies. I Quantitation of subsets according to diagnosis and sites of accumulation and demonstration and counts of muscle fibres invaded by T cells. Ann Neurol 16, 193-208. 

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