Antibodies Reagin

Immunoglobulin E igE 190000 <0.0003 [<100] Antibodies (reagines) Reaginic hypersensitivity reactions Parasitic infestations Non-lgE myeloma Congenital deficit Acquired immunodeficiencies... 

Retrospectively, the tool that provided the key to identification of IgE was the preparation of polyclonal antibodies specific for IgE. Using the antibodies, reagin could be identified in vitro and IgE could eventually be isolated from the seram of atopic individuals (6). The anti-IgE antibodies were also employed to identify the first and second cases of IgE myeloma proteins (7) and to establish that IgE represents a unique immunoglobulin isotype (8). 

Diagnostic procedures include dark-field microscopy12, non-treponemal exams10 (i.e., the Venereal Disease Laboratory and the rapid plasma reagin test), and treponemal exams (i.e., enzyme immunoassay, the T. pallidum hemagglutination test, the fluorescent treponemal antibody test, and the enzyme-linked immunosorbent assay). 

IgE <0.0001 Reaginic antibody involved in immediate hypersensitivity antihelminthic does not bind complement... 

Lee WY, Sehon AH Abrogation of reaginic antibodies with modified proteins. Nature 1977 267 618-620. 

Late antibodies Mucosa-protecting antibodies Early antibodies B-lymphocyte receptors Reagins... 

Treponemal antibodies and reagins are true immunoglobulins consisting mainly of IgM, but also of IgG. IgM appears early in the infection. 

Nielson, K. H. 1977. Bovine reaginic antibody. III. Cross reaction of anti-human IgE and antibovine reaginic immunoglobulin antisera with sera from several species of mammals. Can. J. Comp. Med. 41, 345-348. 

Ishizaka K, Ishizaka T. 1967. Identification of gamma-E-antibodies as carrier of reaginic activity. J Immunol. 99 1187-1198. 

Anaphylactic Reactions Free antigen Reaginic antibody (IgE) fixed to Degranulation of cell and release... 

ACE = angiotensin-converting enzyme ANA = antinuclear antibody ANCA = antineutrophil cytoplasmic antibody BUN = blood urea nitrogen CBC = complete blood count ELISA = enzyme-Mnked immunoassay assay ESR = erythrocyte sedimentation rate FTA-ABS = fluorescent treponemal antibody absorption HLA = human lymphocyte antigen MHA-TP = micro-hemagglutination-7re/ OMew pallidum-, PPD = purified protein derivative RPR = rapid plasma reagin VDRL = venereal disease reference laboratory. 

An atopic person has reaginic antibodies directed against common environmental allergens, and since immunoglobulin E (IgE) is the most important reaginic antibody in man its measurement is relevant to both clinical allergists and immunologists. 

Cromoglicate inhibits mast-ceU degranulation and histamine release induced by phosphohpase A2, but does not interfere with the interaction of antigen and reaginic antibodies. Evidence is accumulating that it has an important stabilizing action on leukocytes, apart from mast cells, such as neutrophils, eosinophils, and monocytes, and that it also affects nerve reflexes in the lung (2). 

Some people, however, find such reactions a great nuisance. These may be those who have an imbalance, e.g., a poor vocabulary of IgG and IgA antibodies with excessive production of IgE (K2). The object of densensitization courses of immunization in these patients is to try and increase the response in IgG antibodies which would have higher binding affinities and compete effectively to deprive IgE sites of antigen. Because IgE normally has a very low serum level (250 pg/100 ml), any increase in reaginic antibody will be readily seen as an increase in total... 

Fig. 6. Diagram to show how, when allergen becomes bound to mast-cell fixed reaginic antibody (IgE), the vasoactive amines mediating immediate hypersensitivity are discharged. The mast cell is capable of becoming recharged, etc. Excess IgG-antibodies can block the access of allergen, which acts only when bound to two IgE molecules.

Sung CP, Baker AP, Holden DA, Smith WJ, Charkin LW. Effect of Angelica polymorpha on reaginic antibody production. J Nat Prod 1982 45 398 106. 

PARISH, W.E. (1972) Eosinophilia cutaneous eosinophilia in guinea pigs mediated by passive anaphylaxis with IgGl or reagin, and antigen-antibody complexes its relation to neutrophils and mast cells. Immunology, 23, 19-34. 

REVOLTELLA, R. OVARY, Z. (1969) Reaginic antibody production in different mouse strains. Immunology, 17,45-54. 

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