Anion-exchange columns Hamilton

Separation on anion-exchange column (Hamilton PRP-XlOO) with 20 mmol L NH4H2PO4 (pH 5.6) mobile phase and a column temperature of 40 °C [separation time of seven As species 16 min As(V) and the ribose with the glycerol aglycone eluted in the dead volume] on-line ICP-MS detection... 

Enzymatic hydrolysis (trypsine) carried out for fauna samples sediments were treated with 1 mol L H3PO4 in an open focused MW system separation on anion-exchange column (Hamilton PRP-XlOO) step gradient elution with a phosphate-based mobile phase, pH 6-7.5 on-line ICP-MS... 

MMA and DMA separated on anion-exchange column (Hamilton PRPX-100) with phosphate mobile phase lOmmol L. pH 6 on-line ICP-MS... 

A good general-purpose anion-exchange column for non-suppressed applications is the Hamilton PRP-XlOO column. As the selection of eluents used with this column is quite varied, the reader is referred to Hamilton column literature for specific eluent recommendations. The Hamilton PRP-XlOO column is most likely prepared via chemical modification of a porous polymeric substrate. 

Figure 18.3. Separation and quantification of anionic As species by HPLC-ICP-MS using a Hamilton PRP X-100 anion exchange column, 20 mM NH4H2PO4, pH 5.6, 1.5 ml min-1, 40° C.

Gailer, J., Irgolic, K.J. The ion-chromatographic behavior of arsenite, arsenate, methyl-arsonic acid and dimethylarsinic acid on the hamilton PRP-XlOO anion-exchange column. Appl. Organomet. Chem. 8, 129-140 (1994)... 

Enzymatic hydrolysis (protease XIV) followed by anion-exchange separation (Hamilton PRP-XlOO column) in phosphate gradient (from 10 to 100 mmol pH 7) in the presence of 2% (v/v) of methanol and SSID-ICP-MS with octapole reaction system (separation time 15 min)... 

Anion-exchange chromatography with a 30 mM sodium phosphate buffer solution at pH 6 was used for the separation of arsenous acid, DMA, MA, and arsenic acid on the polymer-based Hamilton PRP-XlOO anion-exchange column... 

J Gailer, S Madden, WR Cullen, M Bonner Denton. The separation of dimethylarsinic acid, methylarsonous acid, methylarsonic acid, arsenate and dimethylarsinous acid on the Hamilton PRP-XlOO anion exchange column. Appl Organomet Chem 13 837-843, 1999. 

Figure 3 Separation of multispecies standard of As lll), DMA(V), MA(V), and As V) on a short strong anion exchange column (150 mm PRP X-100, Hamilton, 30 mol 1 phosphate buffer adjusted with ammonia to pH 6.0). Concentration of each species 5,10, 15, and 30 ng arsenic per liter.

Armed with several anion-exchange columns and an eluent solution of 2.0 mM KHP (pH 5.0), Siriraks et al. set out to determine why the elution order they observed (Zn-Pb-Cu) was different than that reported by Jenke and Pagenkopf [20]. They studied the chromatographic behavior of Pb(II), Zn(II), and Cu(II) on four different anion-exchange columns in an attempt to elucidate the mechanism of retention for the three cations. The following columns were used in the study silica-bonded from Vydac (302.IC) polystyrene-divinylbenzene (PS-DVB) from Hamilton (PRPX-100) pol)miethacrylate from Waters (IC-Pak) and a latex agglomerated PS-DVB from Dionex (AS-4). 

Most methods for analysis of these compounds are reversed-phase separations on Cg or Cig packings, although anion exchange columns are occasionally used for lower molecular weight products (see Table 2). Polymer columns such as the Hamilton PRP-1 or Di-onex MPIC may also be used, with somewhat less resolution. An organic or inorganic salt... 

A typical nonsuppressed SCIC separation obtained with a low-capacity resin-based strong anion exchanger (PRP-XlOO Hamilton) used as the analytical column is illustrated in Fig. 2 for the simultaneous determination... 

The Dionex column caused each of the metal ions to be completely retained. The authors reasoned that this behavior was due to the presence of unreacted surface-sulfonated PS-DVB macroparticles which were capable of adsorbing the metal cations. A mixed retention mechanism was theorized for the other three columns. In addition to anion-exchange of negatively charged metal-phthalate complexes, the authors suggest that adsorption of neutral metal-phthalate complexes might contribute to the retention of each metal ion. This theory was based on the fact that since the stationary phase of the Hamilton, Waters, and Vydac columns were comprised of different materials, these materials would have different hydrophobicity which would lead to differences in adsorption of the neutral metal-phthalate complexes on each column. This was corroborated experimentally, as the metal ions were retained more strongly on the more hydrophobic column (PS-DVB from Hamilton). The dominant mechanism of retention was similar for each column because there were no differences in metal ion elution orders between the three columns. 

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