Analysis of Microorganisms

D. C. White, Analysis of microorganisms in terms of quantity and activity in natural environments. Microbes in their natural environments, Soc. Gen. Microbiol. Symp. 34-. >l (1983). 

This chapter will address the applications of protein-based bioinformatics to analysis of microorganisms introduced intact into the instrumental system for rapid processing and analysis. Strategies that require offline extraction and fractionation of proteins will not be discussed. Although the amplification of nucleic acids is a powerful approach, especially coupled with mass spectrometry,15 it requires extraction and processing, and thus is not included. 

The analysis of microorganisms in mixtures provides a continuing analytical challenge, for which a number of solutions have been proposed ... 

Bundy, J. L. Fenselau, C. Lectin and carbohydrate affinity capture surfaces for mass spectrometric analysis of microorganisms. Anal. Chem. 2001, 73,751-757. 

Traditionally, the majority of the studies in this area have emphasized the analysis of microorganisms, in particular of soil actinomycetes, as the most relevant source of potential herbicide agents. The plant kingdom, however, has also been explored for the discovery of new herbicides. So far, bialaphos, originally isolated from different Streptomyces strains, is the only commercial herbicide derived from a microbial source. On the other hand, cymethylin, an analog of the mono terpenoid 1,8-cineole, as well as... 

Ward, D. M., M. M. Bateson, R. Weller, and A. L. Ruff-Roberts. 1992. Ribosomal RNA analysis of microorganisms as they occur in nature. Advances in Microbial Ecology 12 219-286. 

The first step of automated image analysis of microorganisms is the visualization. Considering their size range, microscopes (or for the largest objects, microviewers) have to be used. Two aspects should be discussed ... 

Reports on a chemical analysis of microorganisms by infrared spectroscopy, including ideas to use it for identification, go back to the 1950s, e.g. [77]. Due to the poor reproducibility between instruments and problems with evaluating complex spectra, not much development followed these early works for a long time. 

Clesceri, L.S., The Use of Nucleic Acid Probes and Other Molecular Tools in the Functional Analysis of Microorganisms in Soil and Water , in Ecoloxicology Afom toriwg, Richardson, M.L. (Ed.), VCH Publishers, Weinheim, 1993, pp. 163-171. 

Logistic Approach to Analysis of Microorganism Growth in a Batch Bioreactor... 

A summary of experimental conditions for selected analytical pyrolysis studies of microorganisms is given in Table 9.1. The literature review included here is not intended to be comprehensive but is designed to summarize selected recent applications of analytical pyrolysis to the analysis of microorganisms. 

Direct analysis of microorganisms, including food poisoning microorganisms, has also been conducted by MALDI-TOF MS. This promising technique can be used to characterize bacteria rapidly by genus, species, and strain. 

Fig. 2.1 Relationship between sample preparation time and complexity for several MS-based methods for the analysis of microorganisms...

Sample Preparation Methods for the Rapid MS Analysis of Microorganisms... 

Birmingham J, Demirev P, EIo Y-P, Thomas J, Bryden W, Eenselau C. Corona plasma discharge for rapid analysis of microorganisms by mass spectrometry. Rapid Commun Mass Spectrom. 1999 13(7) 604-6. doi 10.1002/(SICI)1097-0231(19990415)13 7<604 AID-RCM529>3.0. 

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