Analysis by mass spectrometry

Ppa-Y-K-APRPPRV Boc-E-Ng-Y-K-APRPPRV Boc-P-E-/Vg-Y-K-APRPPRV Lau-P-E-/Vg-Y-K-APRPPRV Boc-D-Tm-P-E-A/g-Y-K-APRPPRV Fmoc-D-Tm-P-E-A/g-Y-K-APRPPRV 

---

Table 5.5 Nomenclature of the ions formed in the mass spectral fragmentation of polypeptides. From Chapman, J. R. (Ed.), Protein and Peptide Analysis by Mass Spectrometry, Methods in Molecular Biology, Vol. 61, 1996. Reproduced by permission of Humana Press, Inc. 

J. R. (Ed.), Protein and Peptide Analysis by Mass Spectrometry, Methods in Molecular Biology, Vol. 61, 1996. 

A final problem for bioinformatics and bioanalytical scientists is the characterization of engineered microorganisms. Whole-cell analysis by mass spectrometry has been used to confirm the introduction of therapeutic genes into adenovirus vectors,100 to confirm the expression of recombinant proteins in bacteria,101,102 and also in vaccinology.103 In the broader case, identification of... 

Joubert-Caron R et al. Protein analysis by mass spectrometry and sequence database searching a proteomic approach to identify human lymphoblastoid cell line proteins. Electrophoresis 2000 21 2566— 2575. 

R. Boyd, R. Bethem, C. Basic and D. Matthews, Trace Quantitative Analysis by Mass Spectrometry, J. Wiley Sons, New York, 2008. 

Leitner, A., and Lindner, W. (2004) Current chemical tagging strategies for proteome analysis by mass spectrometry./. Chrom. B813, 1-26. 

J. C. Vickerman and D. Briggs (eds.), TOF-SIMS Surface Analysis by Mass Spectrometry. Huddersfield, UK, IM Publications and Surface Spectra Limited, 2001. 

Ligon, W.V., Jr. Molecular Analysis by Mass Spectrometry. Science 1979, 205, 151-159. 

Kondrat, R.W. Mixture Analysis by Mass Spectrometry Now s the Time. Int. J. Mass Spectrom. 2001, 212, 89-95. 

Min, D.H., Yeo, W.S., Mrksich, M. A method for connecting solution-phase enzyme activity assays with immobilized format analysis by mass spectrometry. Anal. Chem. 2004, 76, 3923-3929. 

Proteome analysis by mass spectrometry. Annu. Rev. Biophys. Biomol. Struct. 2003, 32, 399-424. 

R.L. Protein surface mapping by chemical oxidation structural analysis by mass spectrometry. Anal. Biochem. 2003, 313, 216-225. 

Ahearn, A. J., ed., Trace Analysis by Mass Spectrometry. Academic Press, New York, 1972. 

Peptide bonds are cleaved in a nonselective, but not in a completely random manner. Based on anchimeric side-chain assistance, steric factors, and bond strains, acid-labile peptide bonds are predicted to include sites containing Asp, Glu, Ser, Thr, Asn, Gin, Gly, and ProJ22l The disulfide topologies of circulin B and cyclopsychotride, backbone-cyclized peptides with three disulfide bonds, were determined by partial hydrolysis for 5 hours.[22 Occasionally, the bond between adjacent half-cystine residues is cleaved due to the nonselective nature of the mechanism of partial acid hydrolysis.[21] By this procedure, in all cases, a complex mixture of peptide fragments is produced which requires careful chromatographic separation by RP-HPLC for subsequent analysis by mass spectrometry (see Section 6.1.6.2.7). 

Griffin, T.J., Goodlett, D.R., Aebersold, R. (2001). Advances in proteome analysis by mass spectrometry. Curr. Opin. Biotechnol., 12(6), 607-612. 

Coupling chromatographic procedures with immunochemical techniques can also provide a very sensitive and specific analytical system for either determinative or confirmatory analysis. If the antibody used is very specific for the analyte of interest and the antibody reactivity is known to be sensitive to small variations in the structure of the analyte tested, positive reactions with the method are strongly indicative that an analyte of defined structural characteristics is present in the sample. Full rigorous confirmation, however, would depend on further analysis by mass spectrometry, which is the method of choice in confirmatory analysis. Mass spectrometry gives specific information on the identity and structure of the compound of interest. Coupled with chromatographic techniques it becomes a very powerful confirmatory tool for both quantitative and qualitative assessment of drug residues in foods. 

---