The Analysis of Antihistamine Drugs by Gas Chromatography-Mass Spectrometry

The Analysis of Antihistamine Drugs by Gas Chromatography-Mass Spectrometry 

Gas chromatography-mass spectrometry Critical thinking application 

The use of gas chromatography-mass spectrometry (GC-MS) as an analytical technique is growing in importance. GC-MS is a powerful technique in which a gas chromatograph is coupled to a mass spectrometer that functions as the detector. If a sample is sufficiently volatile to be injected into a gas chromatograph, the mass spectrometer can detect each component in the sample and display its mass spectrum. The user can identify the substance by comparing its mass spectrum with the mass spectrum of a known substance. The instrument can also make this comparison internally by comparing the spectrum with spectra stored in its computer memory. 

In this experiment, you will prepare solutions of common over-the-counter antihistamine and cold-remedy tablets. The samples, once prepared, will be analyzed using a GC-MS instrument, and you will use the results to identify the significant antihistamine substances that comprise the original tablet. 

This experiment requires the use of a GC-MS instrument. Before using this instrument, be certain to obtain instructions on its operation. As an option, your instructor may choose to perform the injections. 

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