Amyloglucosidase Activity

Amyloglucosidase Activity), 798 Glucose Isomerase Activity, 796 Glucose Oxidase Activity, 798 P-D-Glucosidase Activity, 798 Hemicellulase Activity, 799 Invertase Activity, 800 Lactase (Acid) (P-Galactosidase) Activity, 802... 

Wu X and Zhou W 1991 Fermentation monitoring and control by on-line flow injection and liquid chromatography Anal. Chim. Acta 249 87-100 [ 16] Holm K A 1986 Automated spectrophotometric determination of amyloglucosidase activity using p-nitrophenyl-a-D-glycopyranoside and a flow injection analyzer Analyst 111 927-9... 

Amyloglucosidase removes consecutive glucose molecules from starch and some other compounds. Reactions [VI] and [VII] are also suitable for detection of amyloglucosidase activity. The best method is generally to determine the rate of glucose formation. 

One unit of amyloglucosidase activity is defined as the amount of enzyme that releases 1 mg of glucose from starch in 1 min at pH 4.5 and 55 C. 

Glucose syrups are also transformed by enzymatic transglucosy-lation (using amyloglucosidase activity) to gentiooligosaccharides... 

Removal of starch impurities About lOOmg of the extracted pectic fractions were dissolved in 30ml of NaOH 0.05M and stirred for 20h at 0°C. The solutions were neutralised by the addition of 0.18ml glacial acetic acid and the pH was adjusted to 4.6. After the addition of I ml of an amyloglucosidase solution (60U/ml) without any detectable side activities, the samples were incubated at 60°C for 3h. Finally, the samples were cooled to room temperature, dialysed (cut-off 12000D) against deionised water (4 C) for 72h and freeze-dried. 

Starch Enzymatic Amyloglucosidase, mutarotase, glucose oxidase and catalase co-immobilised on either nylon net or activated collagen membrane Vrbova et al. (1993)... 

Including similar activities, amylase, amyloglucosidase, not precisely differentiated Fermentation with the meaning of microbial activity... 

In a second example, Storey et al. demonstrated that one could covalently immobilize amyloglucosidase using hydrophilic prepolymers. A 5-mg/ml solution of the enzyme was mixed with an equal volume of prepolymer. The method was judged superior as a support for enzyme immobilization. The percent activity inuno-bili/ed in the polyurethane foams was 25 1.5%. 

Carbohydrase (Aspergillus oryzae var.) Produced as an off white to tan, amorphous powder or a liquid by controlled fermentation using Aspergillus oryzae var. Soluble in water (the solution is usually light yellow to dark brown), but practically insoluble in alcohol, in chloroform, and in ether. Major active principles (1) a-amylase, (2) glucoamylase (amyloglucosidase), and (3) lactase. Typical applications used in the preparation of starch syrups, alcohol, beer, ale, bakery products, and dairy products. 

Preparation and comparison of catalytic activity of immobilized Amyloglucosidase with native enzyme on dextrin - hydrolysis... 

To determine the catalytic activity of chitin-immobilized Amyloglucosidase on the hydrolysis of dextrin, an experiment was conducted in which rate of hydrolysis of dextrin was monitored with the increase of time. To lOOmg of dextrin 2 units of chitin-immobilized... 

---