Amoxicillin fluorescence detection

WH Luo, EB Hansen, CYW Ang, J Deck, JP Freeman, HC Thompson. Simultaneous determination of amoxicillin and ampicillin in bovine milk by HPLC with fluorescence detection. J Agr Food Chem 45 1264-1268, 1997. 

Mascher, H. Kikuta, C. Determination of amoxicillin in plasma by high-performance liquid chromatography with fluorescence detection after on-line oxidation. J.Chromatogr., 1990, 506, 417-421... 

FSIS laboratories also use chemical techniques and instrumentation to identify select antibiotic residues. The tetracyclines of interest are identified by thin layer chromatography. Sulfonamides are detected and quantified by fluorescence thin lay chromatography and confirmed by gas chromatography/mass spectrometry. Amoxicillin and gentamycin are identified and/or quantified by high pressure liquid chromatography. Similar techniques are used to identify ionophores and other antimicrobials of interest. 

Fluorometric detection has been mainly employed for the determination of aminopenicillins such as amoxicillin and ampicillin in edible animal products because it confers the advantages of selectivity and sensitivity. Fluorometric detection of penicillins, however, necessitates their precolumn derivatization to produce the corresponding fluorescent derivatives. The most commonly used derivatizing reagents are formaldehyde (100, 117, 118), salicylaldehyde (83), and mercury dichloride (91). 4-Bromomethyl-7-methoxycoumarin has also been employed as a fluorescence label for the selective and sensitive detection of seven penicillins in milk (96). 

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