Ammonium acetate mixed

Sample preparation Condition a 1 mL cyano Bond Elut SPE cartridge with 2 mL MeOH and 2 mL 10 mM pH 5.0 ammonium acetate buffer. 1 mL Plasma + 1 mL 200 mM ammonium acetate, mix, add a 1 mL aliquot to the SPE cartridge, wash with 2 mL 10 mM pH 5.0 ammonium acetate, wash with 1 mL MeOH 10 mM pH 5.0 ammonium acetate 20 80, wash with 1 mL hexane, dry under vacuum for 1 min, elute with 2 mL MeCN triethylamine 100 0.1, evaporate the eluate to dryness under a stream of nitrogen at 30°, reconstitute with 200 xL mobile phase, vortex for 30 s, inject a 50 p.L aliquot. 

Sample preparation Condition a 3 mL 7 mm Empore Mixed Phase Cation-MPC SPE disc with 1 mL MeOH, three 3 mL portions of MeOH 50 mM ammonium acetate 90 10, and 1 mL 5 mM pH 3.5 ammonium acetate. Mix 100 p,L plasma or urine with 50 xL IS solution and 1 mL 5 mM pH 3.5 ammonium acetate buffer, add to the SPE disc, wash with 1 mL water, wash with 1 mL MeOH, wash with 1 mL MeOHiwater 90 10, dry under vacuum, elute with 1 mL MeOH 50 mM ammonium acetate 90 10. Evaporate the eluate to dryness under a stream of nitrogen at ca. 50°, reconstitute the residue with 150 iiL water, centrifuge at >500 rpm, inject a 100 p,L ahquot. (IS solution contained 50 ng/ml of ds-oseltamivir and 2 fig/ml of d3-GS4071 in water). 

In a flask the chemist mixes 50g piperonal into 200mL glacial acetic acid, then adds 45mL nitroethane and 17g ammonium acetate. The solution is then refluxed 4 hours and takes on the color of yellow to yellow-orange. After 4 hours and cooling, yellowish crystals of p-nitropropene will spontaneously form. If not, the solution can be diluted with 50ml of dHjO and chilled in an ice bath for an hour to form the crystals with some slushy glacial acetic acid and water intermixed. The mass of crystals is broken up and plopped into a Buchner funnel to be vacuum filtered. The filter cake is washed with a little extra acetic acid or water. All of the filtrate is saved. 

Buffer solution, concentrated. Dissolve 27.5 g ammonium acetate and 11.0 g hydrated sodium acetate in 100 mL water add 1.0 mL glacial acetic (ethanoic) acid and mix well. 

Thermospray ionisation sources are usually outfitted with a quadrupole or magnetic sector mass spectrometer (including hybrids or tandem forms). Thermospray operation allows a reversed-phase solvent system, e.g. a 50 50 (v/v) water-methanol or acetonitrile mix containing 0.1 M ammonium acetate. This ensures compatibility with the universal HPLC procedures available in many industrial research laboratories. 

Mobile phase Dissolve 5 g of ammonium acetate in 200 mL of water, add 300 mL of acetonitrile and 500 mL of methanol, mix, filter, and degas. Make adjustments, if necessary (see system suitability under Chromatography <621 >). 

Various chromogenic reagents have been used for the spectrophotometric determination of boron in seawater. These include curcumin [108,109], nile blue [110], and more recently 3,5 di-tert butylcatechol and ethyl violet [111]. Uppstroem [108] added anhydrous acetic acid (1 ml) and propionic anhydride (3 ml) to the aqueous sample (0.5 ml) containing up to 5 mg of boron per litre as H3BO3 in a polyethylene beaker. After mixing and the dropwise addition of oxalyl chloride (0.25 ml) to catalyse the removal of water, the mixture is set aside for 15-30 minutes and cooled to room temperature. Subsequently, concentrated sulfuric-anhydrous acetic acid (1 1) (3 ml) and curcumin reagent (125 mg curcumin in 100 ml anhydrous acetic acid) (3 ml) are added, and the mixed solution is set aside for at least 30 minutes. Finally 20 ml standard buffer solution (90 ml of 96% ethanol, 180 g ammonium acetate - to destroy excess of protonated curcumin - and 135 ml anhydrous acetic acid diluted to 1 litre... 

Aliquots (0.25 mL) of plasma samples were mixed with 50 pL of the IS (Pfizer Global UK-115794, 20 /ig/mL in water) followed by 0.5 mL 0.2M ammonium acetate buffer (pH 9.0), extracted with 7 mL ethylacetate diethylether (1 1 v/v), vortexed for 90 sec, centrifuged at 1500 g for 3 min, and frozen. The organic layer was collected, evaporated to dryness at 40°C under a stream of nitrogen, reconstituted with 0.2 mL of mobile phase, and centrifuged at 10,000 g for 6 min. The supernatant was collected and assayed. The injection volume was 30 pL. Figure 11.2 shows chromatograms of voriconazole and the IS in plasma. 

Blank, calibrator, control, and patient whole-blood samples (50 /iL) were transferred into 1.5 mL conical test tubes, mixed with 100 /xL of the IS, vortexed for 10 sec, and centrifuged at 13,000 g for 5 min. Twenty-five microliters of supernatant were injected onto a Cohesive Technologies Cyclone polymeric turbulent flow column (50 x 1 mm, 50 /flushed with a mixture of methanol and water (10 90 v/v) at a flow of 5 mL/min. Column switching from the TFC to HPLC systems was via a Cohesive Technologies system. The analytical column was a Phenomenex Phenyl-Hexyl-RP (50 x 2.1 mm, 5 /.mi). The mobile phase consisted of methanol and ammonium acetate buffer (97 3 v/v). The buffer was 10mM ammonium acetate containing 0.1% v/v acetic acid. The flow rate was 0.6 mL/min. 

Soil samples were collected along a traverse over the Honerat kimberlite and extended off the kimberlite approximately 75 m SE and 225 m NW from the pipe s centre (Fig. 1). Although it is common practice to collect samples from upper B-horizon soil (Levinson 1980 Bajc 1998 Mann et al. 2005) our samples were collected from C-horizon soil because GAGI samplers were placed at a depth of 60 cm (well below the B horizon). Within 8 hours of sampling, a portion of each soil sample was mixed with Milli-Q water (1 1) to create a slurry. The values of pH and oxidation-reduction potential (ORP) were determined in each slurry. Ammonia acetate leach of the soil samples were performed at Acme Analytical Laboratories, Vancouver, where 20 ml of ammonium acetate was mixed with 1 g soil sample and elements were determined by inductively coupled plasma-mass spectrometry. The GAGI samplers installed at Unknown were placed in piezometers and submerged in water at a depth of approximately 1 m below ground surface. 

Column (10) Indicates that volatile material Is aspirated into the huhhler C containing a 10 per cent aqueous solution ot ammonium molybdate mixed with an equal volume of benzidine solution (made by dissolving 0 05 g. of benzidine in 10 ml. of glacial acetic acid and making up to 100 ml. with water). D.F.P. and tabun are hydrolysed to phosphate and a typical green or green-blue coloration is produced.1... 

All reagents and solvents were used as received (Sigma-Aldrich) if not stated otherwise. NOL-130 stab initiation mix and preformed, 19 mg graphite-blended l,3,5-trinitro-l,3,5-triazacyclohexane (RDX) pellets were purchased from Day Zimmerman (DZI). Samples prepared for LC-MS analysis were dissolved in ammonium hydroxide and run utilizing a Phenomenex Gemini C6-Phenyl column (4.6 mm X 150 mm) and a mobile phase of 20 mM ammonium acetate in water pH 7.02 at 0.75 ml,-min The HPLC analysis is done using... 

Another study employed an ODS column and different mobile phase composition for the measurement of carotenoids in orange juice. Citrus fruits were hand-squeezed and the juice was filtered. Aliquots of 5 ml of juice were extracted with ethyl acetate (3 X 50 ml) containing 0.004 per cent butyl hydroxytoluene (BHT). The organic phase was dried with 50 g of anhydrous sodium sulphate and the aqueous phase was mixed with 50 ml of mehanol and 100 ml of 1 M NaCl, extracted with 75 and 25 ml of ethyl acetate. The ethyl acetate fractions were combined, evaporated to dryness at 40°C and redissolved in the mobile phase. Extracts were analysed in an ODS column (250 X 4.6 mm i.d. particle size 5 jian). The mobile phase consisted of ACN-methanol-l,2-dichloroethane (60 35 5, v/v) containing 0.1 per cent BHT, 0.1 per cent triethylamine and 0.05 M of ammonium acetate. The column was not thermostated and the flow rate was 1 ml/min. Pigments were detected... 

The first of these is a 0.01 M aqueous solution of sodium metaperiodate. The second is prepared by dissolving 0.25 g of stannic chloride decahydrate in 100 mL of IN hydrochloric acid (this reagent must be prepared fresh before use). The third reagent is a 4% solution of ethyl acetoacetate in 20% aqueous ammonium acetate. To run the method, 0.2 mL of the sodium metaperiodate reagent is mixed with 1 mL of the sample dissolved in 2% ethanol, and allowed to let stand at room temperature for 20 minutes. After that, one adds 0.8 mL of the stannic chloride reagent,... 

Sixty grams of ammonium acetate and 40g of sodium nitrite are added to 50ml of water the stirred mixture is carefully warmed to 25-30°C. When solution is complete, the clear, rather viscous liquid is mixed well with the am-moniacal nickel solution and allowed to stand at room temperature. Precipitation of the red complex, which soon begins, is complete after about one hour. When the preparation has stood overnight, the faintly-colored viscous mother liquor is carefully decanted and the salt is brought onto the filter with 95% alcohol. It is washed with 50ml more of the solvent (both sodium nitrite and ammonium acetate are soluble) and dried in air. 

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