7-Amino-4-methylcoumarin-3-acetic acid AMCA

Succinimidyl-7-amino-4-methylcoumarin-3-acetic acid (AMCA-NHS) is an amine-reactive derivative of AMCA containing an NHS ester on its carboxylate group (Pierce). The result is directed reactivity toward amine-containing molecules, forming amide linkages with the AMCA fluorophore (Fig. 221). Proteins labeled with AMCA show little to no effect on the isoelectric point of the molecule. 

High-performance size-exclusion chromatography (HPSEC) was used for competition studies. Solubilized HLA-DR1 (0.13 pM) was incubated for 48 h at 37 °C with the N-ter-minally 7-amino-4-methylcoumarin-3-acetic acid (AMCA)-labeled IM-(19-31)-peptide dissolved in 150 mM sodium phosphate, pH 5.5, containing 15 % (v/v) acetonitrile, 0.1 % (w/v) Zwittergent-12 (Calbiochem) and a cocktail of protease inhibitors (0.2 mM PMSF, 5 pM leupeptin, 10 pM pepstatin, and 1 pM chymostatin). Competition assays were performed in a 1.5 pM solution of AMCA-peptide as competitors, different peptides, the peptide library or peptide sublibraries were added in concentrations ranging from... 

AMCA-Sulfo-NHS Sulfosuccinimidyl-7-amino-4-methylcoumarin-3-acetic acid MW 431... 

Abbreviations used FITC = fluorescein isothiocyanate DTAF = dichloro-triazinylaminofluorescein, TRITC = tetramethylrhodamine isothiocyanate, RB-200SC s lissamine rhodamine sulfonylchloride, RBITC = rhodamine B isothiocyanate, XRITC = rhodamine X isothiocyanate CY3.18 = cyanine 3.18, B-PE = B phycoerythrin, R-PE = R phycoerythrin SITS = 4-acetamido-4 -isothiocyanatostilbene-2,2 -disulfonic acid, DAMC = diethylaminocoumarin, AMCA = 7-Amino-4-methylcoumarin-3-acetic acid. Information obtained from refs. (2,9,10)... 

Other Names 2//-l-Benzopyran-6-sulfonic acid, 7-amino-3-[2-[(2,5-dioxo-l-pyrroiidinyl)oxy]-2-oxoethyl] -4-methyl-2-oxo- 7-amino-3-((((succinimidyl)oxy)-carbonyl)methyl) -methylcoumarin-6-sulfonic acid AMCA-S Alexa 350 carboxylic acid succinimidyl ester Alexa Fluor 350 carboxy acid succinimidyl ester Alexa Fluor 350 succinimidyl ester Alexa Fluor 350NHS Sulfosuccinimidyl-7-amino-4-methylcoumarin-3-acetic acid... 

Alexa Fluor 350 carboxylic acid succinimidyl ester (AMCA-S) 7-Amino-4-methylcoumarin (AMC) (Coumarin 120) (Coumarin 440) 7-Anilinocoumarin-4-acetic acid (ACAA)... 

---