Amino acids deletion

Fig. 3. Fluman LH, FSH, and TSH a suburnt [69431-84-1]. Amino acid numbering is relative to maximum homology between species (48). Note the 4 amino acid deletion in human a suburnt between positions 6 and 9. Consensus glycosylation sites are at Asn-56 and 82. GHO = carbohydrate chain.

The crystal structure of the HNL isolated from S. bicolor (SbHNL) was determined in a complex with the inhibitor benzoic acid." The folding pattern of SbHNL is similar to that of wheat serine carboxypeptidase (CP-WII)" and alcohol dehydrogenase." A unique two-amino acid deletion in SbHNL, however, is forcing the putative active site residues away from the hydrolase binding site toward a small hydrophobic cleft, thereby defining a completely different active site architecture where the triad of a carboxypeptidase is missing. 

HI 1. Hirono, A., Fujii, H Shima, M and Miwa, S., G6PD Nara Anew class 1 glucose-6-phosphate dehydrogenase variant with an eight amino acid deletion. Blood 82,3250-3252 (1993). 

The carboxy-terminal region in apolipoprotein (a) closely resembles the protease domain in plasminogen [eight amino acid substitutions, nine amino acid deletions, and one insertion in apo(a) relative to plasminogen, with 94% overall nucleotide sequence identity] (G28). The most important difference is the substitution of arginine by serine in the site responsible for proteolytic activity (position 4308) (G28). As a result, Lp(a) has no protease activity towards substrates for plasmin (J3). Salonen (SI) reported a serine-protease activity of Lp(a) towards fibronectin, a glycoprotein present in connective tissue matrices. 

Meanwhile the 3D structure of SbHNL was solved [27], showing the expected high similarity to the structure of GPU from wheat [41]. Both enzymes use the same active-site residues. However, in SfeHNL a unique two-amino acid deletion... 

B)[50], The proteins encoded by the ADH1, ADH2, ADH3, and ADH5 genes also share a 22-amino acid deletion compared to the HLADH sequence. This region is part of a surface loop in HLADH relatively far from the active site. 

For peptide libraries, one solution to this problem uses matrix-assisted laser desorption ionization (MALDI) mass spectrometry to directly desorb and identify peptides from beads that were screened and found to be hits (33). This technique is called the termination synthesis approach. Because the peptide library compounds are analyzed directly, products with amino acid deletions or substitutions, side-reaction products, or incomplete deprotection are readily observed. Also, because there are no extra molecules used for chemical... 

Basani RB, Vilaire O, Shattil SJ, et al. Glanamann thrombasthenia due to a two amino acid deletion in the fourth calcium binding domain of alpha lib Demonstratiem of the importance of calcium binding domains in the conformation of alphallbbeta3. Blood 1996 88 167-173. 

As previously noted, apoA-I contains Pro-punctuated tandem repetitive amphipathic helical domains. In apoE, however, the major lipidbinding domain maps to a class A amphipathic helix motif (residues 202-266 see Fig. 7) with no Pro punctuations (Fig. 12A). Thus, this is by far the longest unbroken amphipathic helix among the exchangeable apolipoproteins (65 residues) and one in which the polar-nonpolar interface is in register throughout its length due to a four-amino-acid deletion compared with apoA-I. 

Small KM, Forbes SL, Rahman FF, Bridges KM, Liggett SB. A four amino acid deletion polymorphism in the third intracellular loop of the human a2C-adrenergic receptor confers impaired coupling to multiple effectors. J Biol Chem 2000 275 23,059-23,064. 

One polymorphic form of the o bAR, consisting of a three-amino acid deletion (denoted Del3 01-303) located within the third intracellular loop of the receptor, has been described (110). This polymorphism consists of an in-frame nine-bp deletion beginning at nucleotide 901 that results in the loss of three Glu residues at positions... 

Heinonen P, Koulu M, Pesonen U, Karvonen MK, Rissanen A, et al. 1999. Identification of a three-amino acid deletion in the a2B-adrenergic receptor that is associated with reduced basal metabolic rate in obese subjects. J. Clin. Endocrinol. Metab. 84 2429-33... 

Sivenius K, Lindi V, Niskanen L, Laakso M, Uusitupa M. 2001. Effect of a three-amino acid deletion in the alpha2B-adrenergic receptor gene on long-term body weight change in Finnish nondiabetic and type 2 diabetic subjects. Int. J. Obes. Relat. Metab. Disord. 25 1609-14... 

For calibration in the second dimension, use is made of proteins of known molecular mass which give horizontal streaks across the final gel which each define a different and known mass (8). Mass differences of greater than 2% may be detected over the entire mass range resolved, which means that single amino acid deletions cannot ordinarily be seen ( ). 

Liu B, Peng D, Lu Y et al. A novel single amino acid deletion caspase-8 mutant in cancer cells that lost proapoptotic activity. J Biol Chem 2002 277 30159-30164. 

After the successful expression and purification of the SslA truncation derivatives, their self-assembling properties and lattice symmetry formation were investigated. Are these derivatives able to self-assemble in vitro or is this ability lost with certain amino acid deletions If self-assembly is preserved, what do the selfassembling structures look like Can the p4 symmetry, as in case of the native SslA, be observed Which factors will influence the self-assembly process To answer all these questions, in vitro recrystallization experiments were conducted on a silicon wafer. 

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