Alkaline Phosphatase ALP Activity

Gao and Yamaguchi, 1999a Yamaguchi and Ma, 2001 Femoral-diaphyseal tissues from elderly female rats cultured for 24 h Daidzein or genistein (lO M, lO M) induced calcium content and alkaline phosphatase (ALP) activity indicating stimulation of bone formation. [Pg.99]

Bilirubin. An increase in the bilirubin level and an accompanying increase in aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities most often result from obstmctive jaundice developed as a consequence of pancreas head edema and pressure exerted on the papilla of Vater (cholestasis). [Pg.54]

Measurement of Alkaline Phosphatase (ALP) Activity. Although a multitude of methods are being used to measure peroxidase, most workers use the following method to measure ALP. The reaction utilizes 1 mg of p-nitrophenylphosphate (Sigma) per milliliter in 1 M diethanolamine buffer, pH 9.8. The reaction is stopped by addition of i volume of 2 M NaOH. The yellow p-nitrophenol is measured at 405 nm. [Pg.433]

Fig. 6. Alkaline phosphatase (ALP) activity of rat MSCs cultured for 14 days on hoUow HA microspheres (106-150 pm) containing different amounts of open HA microspheres.

The association of HA with polyester increased the cellular alkaline phosphatase (ALP) activity and the expression of genes related with the osteogenic differentiation in relation to PLGA without HA scaffolds. In addition, the content of calcium was also increased in PLGA-HA fibers. The study indicates that PLGA-HA scaffolds can be good substrates for bone regeneration. The study also proves... [Pg.173]

Mean values for serum osteocalcin (n = 8) and serum alkaline phosphatase (ALP) activity (n = 8) within a row having different superscripts (rn, n for fet effect x, y for CLA effect A, B, Cfor interaction effect) are significantly different by two-way AN OVA and Duncan s multiple-range test (P< 0.05). The factorial design of dietary treatments allowed for an n = 16 in the statistical analysis of main effects. Results from the reference diet (SBO) were not included in the statistical analysis. [Pg.247]

A number of delivery systems have been developed for the controlled release of bone morphogenic protein-2 (BMP-2). For example, calcimn phosphate cement-based materials have shown the ability to deliver recombinant human bone morphogenic protein-2 (rhBMP-2) to increase alkaline phosphatase (ALP) activity in MC3T3-E1 cells in vitro,and enhance bone formation both ectopically and in an ulna osteotomy model. In addition to cement-based systems, polymeric materials have also been heavily researched as potential delivery vehicles of BMP-2. Saito et al. have developed a temperature sensitive poly(D,L-lactic acid-polyethylene glycol) (PLA-PEG) block copolymer as an... [Pg.436]

Alkaline phosphatase (ALP) activity ALP activity of the supematarrt was meastrred using an ALP B-Test WAKO kit (Wako, Japan) based on the Bessey-Lowry method. ALP activity was normalized by samples total protein production measured with a Bicinchoninic Acid Assay (BCA, BCA Protein Assay KiL Pierce Biotechnology, U.S. A.). [Pg.208]

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