Albumin determination

J2. Jacobsen, J., Binding of bilirubin to human serum albumin. Determination of the dissociation constants. FEBS (Fed. Eur. Biochem. Soc.), Lett. 5, 112-114 (1969). 

Albumin Determination with Dyes and Organic Indicators. 272... 

R23. Rosenfeld, M., and Surgenor, D. M., The hematin binding reaction as a basis for serum albumin determination. J. Biol. Chem. 199, 911-921 (1952). 

Gustafsson, J. E. 1976. Improved specificity of serum albumin determination and estimation of acute phase reactants by use of the bromcresol green reaction. Clinical Chemistry 22 616-622. 

Amino-4,6-dinitrophenol is an important intermediate in the manufacture of colorants, especially mordant dyes. It has also been used as an indicator dye in titrations (yellow with acid, red with alkali) and as a reagent for albumin determination. 

Table Xn. Immunochemical Cross-Reaction of Anti-Bovine Serum Albumin and Heterologous Serum Albumins Determined on Immunoadsorbmits"...

Patra, D. (2010). Synchronous fluorescence based biosensor for albumin determination by cooperative binding of fluorescence probe in a supra-biomolecular host-protein assembly Biosensors and Bioelectronics 25, 1149-1154. 

Zou, H.F. Wang, H.L. Zhang, Y.K. Stereoselective binding of warfarin and ketoprofen to human serum albumin determined by microdialysis combined with HPLC. J. Liq. Chromatogr. Relat. Technol. 1998, 21 (17), 2663-2674. 

Richieri, G.V. Anel, A. Kleinfeld, A.M. Interactions of long-chain fatty acids and albumin Determination of free fatty acid levels using the fluorescent probe of ADIFAB. Biochem. 1993, 32, 7574-7580. 

Fig. 29.32 The structure of human serum albumin determined by X-ray diffraction (PDB code 3JRY). The sites highlighted in ball-and-stick representation are the foeus of problem 29.30.

We have developed the method for quantitative analysis of urinary albumin with CE. A capillary electrophoresis systems Nanophor 01 (Institute of Analytical Instmmentation, Russian Academy of Sciences, Saint-Petersburg) equipped with a UV-detector was used to determine analyte. Separation was achieved using 45 cmx30 p.m I.D. fused silica capillary column with UV-detection at 214 nm. 

The aim of our investigation was the development of the amperometric enzyme immunosensor for the determination of Klebsiella pneumoniae bacterial antigen (Ag), causes the different inflammatory diseases. The biosensing pail of the sensors consisted of the enzyme (cholinesterase) and antibodies (Ab) immobilized on the working surface of the screen-printed electrode. Bovine seiaim albumin was used as a matrix component. 

Protein concentration can be determined using a method introduced by Bradford,4 which utilises Pierce reagent 23200 (Piece Chemical Company, Rockford, IL, USA) in combination with an acidic Coomassie Brilliant Blue G-250 solution to absorb at 595 nm when the reagent binds to the protein. A 20 mg/1 bovine serum albumin (Piece Chemical Company, Rockford, IL, USA) solution will be used to prepare a standard calibration curve for determination of protein concentration. The sample for analysis of SCP is initially homogenised or vibrated in a sonic system to break down the cell walls. 

Edwards-Levy, F., Andry, M. C. Levy, M. C. (1994). Determination of free amino group content of serum-albumin microcapsules. II. Effect of variations in reaction-time and terephthaloyl chloride concentration. International Journal of Pharmaceutics, Vol. 103, 3, (March 1994), pp. (253-257), ISSN 0378-5173 Friend, D. R. (2005). New oral delivery systems for treatment of inflammatory bowel disease. Advanced Drug Delivery Reviews, Vol. 57, 2, (January 2005), pp. (247-265), ISSN 0169-409X... 

The sensitivity of enzyme assays can also be exploited to detect proteins that lack catalytic activity. Enzyme-linked immunoassays (ELlSAs) use antibodies covalently finked to a reporter enzyme such as alkafine phosphatase or horseradish peroxidase, enzymes whose products are readily detected. When serum or other samples to be tested are placed in a plastic microtiter plate, the proteins adhere to the plastic surface and are immobilized. Any remaining absorbing areas of the well are then blocked by adding a nonantigenic protein such as bovine serum albumin. A solution of antibody covalently linked to a reporter enzyme is then added. The antibodies adhere to the immobilized antigen and these are themselves immobilized. Excess free antibody molecules are then removed by washing. The presence and quantity of bound antibody are then determined by adding the substrate for the reporter enzyme. 

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