Affymetrix GeneChip® array

Gene Expression Analysis. Figure 1 Schematic representation of the experimental process of global gene expression analysis using Affymetrix GeneChip arrays. 

High background Affymetrix GeneChip microRNA array... 

Alternative microarray approaches are available that involve the 3 tailing of the miRNA molecule with poly(A), followed by labeling and hybridization. The NCode miRNA microarray platform (Life Technologies) involves the addition of the poly(A) tail to the miRNA prior to ligation of fluorescent dye molecules and subsequent hybridization to the antisense miRNA probes on the microarray chip. In the case of Affymetrix GeneChip microRNA arrays, the poly(A)-tailed miRNAs are labeled with a biotinylated signal molecule. A limitation of hybridization-based microarrays is that because of the small size of miRNAs, the Tm of the probes has a wide range that may diminish specificity and/or sensitivity for miRNAs with a low GC content (10,56). 

Genotyping arrays AmpliChip CYP450 Roche Scientific Drug metabolism Affymetrix GeneChip FDA 2004 Blood... 

Affymetrix 418 Array Scanner Laser scanning microscope with two excitation filters can read Affymetrix GeneChips with a sensitivity of less than 1 fluor/pm. www.affymetrix.com... 

Affymetrix Information on GeneChip arrays—http //www.affymetrix.com/index.affx... 

The patented Affymetrix GeneChip is a very powerful and reliable platform, allowing massively parallel analysis. However, this comes at a cost, that is frequently beyond the budget of most academic research laboratories. Home-made arrays have a much lower density of spots and also tend to be less reliable when compared to the stringent controls applied by Affymetrix. They are, however, much cheaper and can be designed specifically for each particular application. As competing companies try to fill the gap for more affordable array platforms, prices will fall and DNA arrays will find increasing use both in academic and in industrial laboratories. 

Measurements of transcript abundance using the Affymetrix GeneChip platform is based upon probe sets consisting of eleven overlapping 25-mer oligonucleotide pairs arrayed on a silicon substrate. Each probe is arranged... 

There are many protocols and different types of platforms available, e.g. GeneChips from Affymetrix, Illumina Bead Arrays, Arrays from Agilent, Applied Biosystems, GE Healthcare, customized spotted cDNA microarrays etc. the basic procedure for a large-scale measurement of gene expression involves the preparation of total or mRNA from the biological sample(s) under investigation (e.g. candidate tissue) and the hybridization of copied labelled RNA or cDNA to the DNA elements on the array surface (Fig. 1). 

Oligonucleotide arrays [6] In-situ on the surface of the matrix 1 cm by 1 cm slide with approximately 40 000 genes Affymetrix s GeneChip can contain up to 400 000 different oligonucleotides and is the densest array Fluorescent tag labeling fluorophore detector is added after hybridization Passive Affymetrix... 

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