Affinity marker

This approach was followed concurrendy with that of steroids as part of platinum coordination complexes for which the principal target was found to be DNA. Despite many structural modifications, the results were not spectacular [104-106]. Very often these Pt hybrids or their associated SERMs provided no synergy [107], as far as new, useftil properties were concerned. On the other hand, with covalent organometallic complexes, a number of pioneering results made it possible to study their recognition for specific nuclear receptors, and to develop novel affinity markers or suicide substrates. These preferentially targeted key proteins in specific biological functions to the detriment of DNA. 

Among the functionalized alkynes which have been reacted with triruthenium and triosmium clusters, there is 11- -ethynylestradiol. The aim of the studies on these complexes was to obtain high-affinity markers and receptors for this estrogen, which plays an important role in breast cancer. Gomplexes with the alkyne bound in... 

The affinity (Kj values) observed for [ H]MDA and [ HJMDMA binding were similar to the effective doses (i.e., ED50 or K] values) of MDA and MDMA reported for various pre- and postsynaptic monoamine markers, such as serotonin and dopamine release (Johnson et al. 1986), monoamine transport (Steele et al. 1987), and multiple brain, ligand binding sites (Battaglia et al. 1988). 

A number of other issues must now be addressed including determining if the IgG-protein A affinity is sufficiently high to keep the antibody associated with the virus in vivo. The full potential of this approach will also require more detailed characterization of surface markers uniquely associated with different cell types. However, the approach exemplifies the types of technical innovation now being introduced that will make second-generation vectors more suited to their role in gene therapy. 

Many precolumns and trap cartridges for sample clean-up are commercially available. In our experience, a 2 to 3 cm short column with twice the analytical column inner diameter and packed with the same particles performs satisfactorily. An antibody affinity column for selective removal of highly abundant proteins from human serum samples provides better sensitivity for the discovery of low abundance protein markers that may represent revolutionary therapeutic diagnosis and monitoring. 

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