Adhesion to endothelial cell monolayer

Sanderson, P., and Calder, P.C. (1998) Dietary Fish Oil Diminishes Lymphocyte Adhesion to Macrophage and Endothelial Cell Monolayers, Immunology 94,79-87. 

In vitro studies have documented that activation of leukocytes by ANCAs can release factors that are injurious to endothelial cells (52-59). Endothelial injury by ANCA-activated leukocytes requires interplay between multiple cytokine and adhesion molecule ligands and their receptors in the microenvironment at the site of vasculitis (55-59). Overall, in vitro activation of neutrophils by ANCA IgG causes neutrophil adherence to endothelial cells, diapedesis through endothelial monolayers, and release of toxic factors that kill endothelial cells (Fig. 1). Similar events in vivo would result in histologic changes that are characteristic of early acute ANCA-associated vasculitis, including neutrophil margination and infiltration and vascular necrosis. 

Price, E. A., Coombe, D. R. and Murray, J. C. A simple fluorometric assay for quantifying the adhesion of tumor cells to endothelial monolayers. (1995). Clin. Exp. Metastasis 13,155-164. 

A specific demand for a biomaterial surface is its compatibility with organ specific ceUs, which leads to the approach to cell seeding before implantation. Therefore, implants for blood contact may be durably covered with a monolayer of endothelial cells. Thus, a suitable interface between the synthetic carrier material and the individual cells is required [132]. For this reason, a concept based on covalent coupling of ceU adhesion promoters, like fibronectin or the pen-tapeptide GRGDS belonging to the endothelial cell binding domain of fibronectin, at the biomaterial surface is pursued. To achieve this the base polymer has... 

Figure 9.1 Relationship between contact angle and cell adhesion. Human umbilical vein endothelial cells open circles) and HeLa cells closed circles) were allowed to attach to self-assembled monolayers (SAMs) carrying methyl (CH3), hydroxyl (OH), carboxylic acid (COOH), or amino (NH2) terminal functional groups mixed with two kinds of alkane thiols (CH3/OH, CH3/COOH and CH3/NH2) for 1 h. A clear trend showing increasing cell adhesion with decreasing contact angle was observed for aU SAMs.

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