Adenosine triphosphate assay

Luciferase assay. In this technique, firefly luciferase is used to measure small amounts of adenosine triphosphate (ATP) in a bacterial culture, ATP levels being reduced by the inhibitory action of aminoglycoside antibiotics. This method may find more application in the future as more active and reliable luciferase preparations become available. 

Enzymatic Assay. The enzymatic (luciferase) assay for adenosine triphosphate (ATP) is one of the methods applied to areas of biocidal control in oil production operation [1454]. A reliable method for the determination of ATP is the measurement of bioluminescence produced by the luciferin luciferase system. 

Before further testing and to confirm that the compounds are cytotoxic rather than merely interfering with the Alamar blue indicator dye, they are re-bioassayed using two other indicator dyes. Calcein-AM is a fluorescent dye that measures changes in cell mem brane permeability, an indicator for one of the penultimate steps of cell death, uci e e measures the amount of adenosine triphosphate (ATP) synthesis in a chemilurmne assay. For some compounds, cell death was also confirmed by microscopic exami Papanicolaou-stained cell preparations.11... 

E. A reliable assay shows that the ATP (adenosine triphosphate) content of a certain cell type is 111 p.mol/100 mL. You developed a new assay, which gave the following values for replicate analyses 117, 119, 111, 115, 120 p.mol/100 mL (average = 116.4). Can you be 95% confident that your result differs from the known value ... 

Enz5une sensors or assays exist for the determination of adenosine triphosphate (ATP) and its degradation products inosine 5 -monophosphate (IMP), inosine (HxR), hypoxanthine (Hx) and xanthine (X) as ATP is used as an indicator of the presence of microorganisms and the concentrations of its degradation products are used as indicators for fish and meat freshness in food industry [118]. 

Living cells generate adenosine triphosphate (ATP) that can readily be detected by enzyme assays, e.g. luciferin emits light when exposed to firefly luciferase in the presence of ATP light... 

ATP Adenosine triphosphate ELISA Enzyme-linked immunosorbent assay... 

Groivth-based technologies—biochemical assays, headspace pressure, adenosine triphosphate (ATP) bioluminescence, colorimetric detection of CO2, and impedance (electrical activity)... 

His research with Grace Picciolo led to his invention of a method for the detection of adenosine triphosphate (ATP). The technique takes advantage of the naturally occurring luciferase enzyme and the chemical lu-ciferin. Both are obtained from the lantern of a firefly hence, this technique is sometimes referred to as a firefly bioluminescent assay. In combination with ATP and magnesium ions, luciferm and luciferase fluoresce, generating a light intensity that is proportional to the amoimt of ATP present. 

Chemiluminescence is also found in fireflies. The male firefly uses the reaction of a luciferin substrate and the enzyme luciferase with oxygen, with adenosine triphosphate (ATP) as an energy source, to create the illumination it uses to attract a mate. Because the detection of very minute amounts of light is possible, chemiluminescence and bioluminescence have become the basis of many sensitive analytical and bioanalytical techniques or assays used to quantify particular compounds in samples. Indeed, the use of these techniques is broad enough to justify the existence of a journal devoted to them, the Journal of Bioluminescence and Chemiluminescence. 

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