Acetic acid preparations

Add 5 ml of sodium hypochlorite (Clorox) to 50 ml of acetic acid prepare the benzidine solution by dissolving 0.5 g of benzidine and one crystal of KI in 50-ml ethanol-HjO (1 1) spray the plates with the Clorox reagent and let dry then spray with benzidine reagent... 

A (Alternative) JCS 3175(1952). 2 g 3-indolyl-acetic acid (preparation given elsewhere here), 1.55 g freshly fused sodium acetate, 5 ml acetic anhydride. Heat 135-140° on oil bath for eighteen hours cool, wash with water and extract with CHCl3-ether (1 4). Wash organic phase with 3X20 ml saturated KHC03 and dry, evaporate in vacuum to get the l-acetyl-3-indolyl-acetone, which can be reduced to the alpha-methyl-tryptophol derivative with lithium aluminum hydride, and then converted to the dialkyl-tryptamine as already described (as can (I)), or used in step B, or reduced to (I) as follows dissolve 1 g in 1 ml 1 N Na-methoxide in methanol and 60 ml methanol, and keep at 40° for 10 minutes acidify with dilute HC1 and extract with ether. Dry, evaporate in vacuum to get (I) (recrystallize-methanol). 

Your instructor has prepared a 50 ppm riboflavin stock solution in 5% (by volume) acetic acid. Prepare a series of calibration standard solutions that are 0.2, 0.4, 0.6, 0.8, and 1.0 ppm from the 50 ppm stock solution and again use 5% acetic acid for the dilution. Use 25-mL volumetric flasks for these standards and shake well. 

Experiments.—In order to learn, at least qualitatively, the influence of the hydrogen ion concentration on the velocity of decomposition, about 0-5 c.c. of ethyl diazoacetate is dissolved in a little 50 per cent alcohol and the solution is divided into two portions in small beakers to which respectively 1 c.c. of 0-1 AT-hydrochloric acid and 1 c.c. of 0-1 N-acetic acid (prepared in a measuring cylinder from glacial acetic acid) are added. 

Fixative Mix three parts methanol with one part glacial acetic acid. Prepare fresh, and keep on ice... 

Acetic acid preparations (usually 2-5% solutions) have both antibacterial and antifungal activities. They are particularly useful against P. aeruginosa Staphylococci, p-hemolytic Streptococci, Candida species, and Aspergillus. No organisms are resistant to these... 

Aqueous acetic acid (preparative procedures), (a) A solution of C1O3 in a small volume of water is diluted with acetic acid the solution is run into a solution of jS-methylnaphthalene in acetic acid and the temperature is controlled to 60° by cooling yield of 2-methyl-1,4-naphthoquinone, 38-42%. ... 

Major end uses for methanol are for the production of formaldehyde, about 30%, which is used for the preparation of phenol-formaldehyde resins. About 20% is used for the production of methyl -butyl ether, which is used as an additive alone, and in blends with methanol as a fuel component. Further uses are for the esterification of terephthalic, and acrylic acids, and for acetic acid preparation, about 10% each. 

Aqueous acetic acid (preparative procedures) [1,145, after (d)]. 

The presence of acrylonitrile in copolymers can also be demonstrated by heating a sample of the dry material in a test tube and testing with indicator paper for the formation of HCN. Prepare the indicator paper hy dissolving 0.3 g copper(ll) acetate in 100 ml water. Impregnate strips of filter paper, and then air-dry them. Just before use, dip the strips in a solution of 0.05 g benzidine in 100 ml 1IV acetic acid (prepared from equal parts of 2IV acetic acid and water). If HCN is formed and passes over the moist paper, the paper turns blue (Careful ). 

In these molecules, several amine groups and one or more carboxylic acid groups are combined to give highly functionalized surfactants. In particular, alkyl amino acetic acids prepared from polyamines have found interest since these amphoterics show microbio-cidal properties (36). An example is dodecyl propylene diamine acetic acid, which is derived from a diamine and chloroacetic acid by carboxymethylation (Figure 15.23). 

Dense and moderately viscous liquids with the odor of camphor and iodoform. Insoluble in water but can be diluted with carbon tetrachloride, benzene, toluene, acetone, diethyl ether, chloroform, aniline and glacial acetic acid. Prepared by passing acetylene into cooled bromine. With a flash point of 335°C, it decomposes on heating at 235°C. Irritating and narcotic. 

Setting Up Have at your disposal 0.5 M stock solutions of anisole, diphenyl ether, and acetanilide in 15 M(90%) aqueous acetic acid and a 0.02 Msolution of bromine in 15 M acetic acid. Calibrate the spectrophotometer to zero absorbance at 400 nm using a solution of the arene substrate in acetic acid. Prepare this solution by using a 2-mL pipet to combine 2 mL of 15 M acetic acid and 2 mL of the stock solution of the substrate arene in a clean and dry cuvette. Stir the solution to ensure homogeneity and place the cuvette in the spectrophotometer. Adjust the absorbance reading of the spectrometer to zero. Record the laboratory temperature. 

A vigorously stirred suspension of dry Pb-thiocyanate in anhydrous acetic acid (prepared by refluxing acetic acid with 5% acetic anhydride for 4 hrs.) treated with dry bromine, left until colorless, Irons-octadec-6-enoic acid added with stirring which is continued for 8 hrs., and the product isolated the next day eri/t/iro-6,7-di(thiocyanato) octadecanoic acid. Y ca. 80%. F. e. s. J. F. McGhie et al., Soc. 1962, 4638. 

Isopropyl mercaptan and tert-hutyl alcohol added to a soln. of HGIO4 in glacial acetic acid (prepared by adding aq. HGIO4 to acetic acid and removing the... 

Solution 71% O-H3PO4 (orthophosphoric add). Mix 5 vol. parts of commercial concentrated (85%) 0-H3PO4 with 1 vol. part of Dl water (deionized water) Preparation Mix 5 vol. parts of (85%) 0-H3PO4 with 1 vol. part glycerin DAB7 and 0.5 —1.0 g/L sodium alkylarylsulfonate (phenysulfonate) 12% NaOH (sodium- hydroxide). Preparation Dissolve 120 g NaOH in 1 L of Dl water 1 % acetic acid. Preparation Add 20 ml glacial acetic acid to 1 L of Dl water... 

Type C Syntheses (N—C6H4—S—C).—Conversion of o-nitroarylthio-acetic acids, prepared from a-mercaptoacetic acid and the appropriate derivative of < -nitro-chlorobenzene, into the corresponding benzothiazolin-2-one (9) can be brought about by treatment with acetic anhydride followed by hydrolysis with base or acid" (see Vol. 3, p. 622). 

Acetic acid Prepare a 10 % stock solution (v/v) and store at room temperature. 

---