Whole-genome amplification

Ghazani AA, Arneson NC, Warren K, et al. Limited tissue fixation times and whole genomic amplification do not impact array CGH profiles. J. Clin. Pathol. 2006 59 311-315. 

Thompson, M. D., Bowen, R. A., Wong, B. Y., et al. (2005) Whole genome amplification of buccal cell DNA genotyping concordance before and after multiple displacement amplification. Clin. Chem. Lab. Med. 43, 157-162. 

Whole blood. DNA from whole blood is one of the most stable and easily obtainable sample sources. The DNA is usually high yield and high molecular weight and suitable for long-term storage. In addition, the DNA is usually suitable for whole-genome amplification. 

Saliva. The extraction of DNA from mouthwash samples provides high-yield, good-quality DNA that is also suitable for whole-genome amplification (23). The advantage of this sample source over blood is that the procedure for collecting samples is noninvasive, and the mouthwash samples are stable at room temperature for extended periods of time. 

Key Words single nucleotide polymorphism array allelic imbalance copy nmn-ber variation whole-genome amplification formalin-fixed and parafiin-embedded tissue... 

The amount of DNA available from tissue samples, especially samples from tumor biopsies, is usually limited. Fortunately, whole-genome amplification has been developed to generate micrograms of DNA for further analysis (52,53). We found similar genotype calls and copy number changes in whole-genome amplified DNA with that using unamplified DNA, as determined by a 10 K SNP array analysis (30). 

Paez JG, Lin M, Beroukhim R et al. Genome coverage and sequence fidelity of phi29 polymerase-based multiple strand displaeement whole genome amplification. Nucleic AcidsRes2QOA,22-.el. ... 

Santella RM (2006) Approaches to DNA/ RNA extraction and whole genome amplification. Cancer Epidemiol Biomarkers Prev 15 1585-1587... 

Whole genome amplification of buccal cell DNA genotyping concordance before and after multiple displacement amplification. Clin Chem Lab Med 43 157-162... 

More recently, whole genome amplification (WGA), multiple displacement amplification (MDA) and other advanced copying techniques have been evolved [72]. 

A great step forward in polynucleotide analysis was made by Mullis [44] in 1986. This was the introduction of the polymerase chain reaction (PCR), which enabled multiple copies of a given DNA sequence to be readily made. This was followed by whole genome amplification (WGA) and other related techniques (Chapter 11.6). 

Bigdeli S, Dettloff RO, Frank CW, Davis RW, Crosby LD. A simple method for encapsulating single cells in alginate microspheres allows for direct PCR and whole genome amplification. PLoS One 2015 10(2) e0117738. 

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