Washing of tissue

Several methods are described in the literature regarding the washing of tissue sections. We describe representative ones here. The authors impression is that the method in Reference 15, and even more so that in Reference 16, are likely to have a stronger degreasing action than that in Reference 9. 

This type of fixation is often used for cryosections, cell smears and cell monolyers, but can not be recommended for fixation of tissue blocks since acetone and alcohols, as opposed to aldehydes, penetrate tissue poorly. Cryosections, cell smears and cell monolyers after short (for 5 15 min) fixation in alcohols or acetone are usually air-dried (for 1 h or overnight), washed in buffered saline and directly subjected to the immunocytochemical analysis. 

Wash pig liver with water and remove as much Wood as possible. Homogenize chopped pig liver in a blendor with the same volume of Soln. A and cool the homogenate to 0 °C. Pour slowly the homogenate to 10 vol. of acetone cooled to -15 °C. Allow to setde at -15 °C and filter the sediment on a Buchner funnel. Wash the tissue twice with three volumes of cold acetone, followed by a wash with cold diethylether. Spread the dehydrated tissue on filter paper, grind up, allow the solvent to evaporate and store it in a desiccator containing anhydrous calcium cWoride at 4 °C. The white liver powder remains stable for months. 

Staging parameters will vary according to manufacturer design layout (see Note 3). Specimen should be washed of all peripheral tissue to ensure that no irrelevant information is image captured. This can potentially confound the information content of the scan (see Note 4). Likewise, staging material should... 

The column-switching system was applied to the determination of STR and DIHS in pork and bovine muscle and kidney. Perchloric acid was used to precipitate proteins and extract analytes from the tissue. The clear supernatant was further cleaned up by an offline SPE on a cation-exchange SPE column. After the washing of the cartridge with water, the analytes were eluted with phosphate buffer (pH 8.0) and diluted with HSA, perchloric acid, and water. The enrichment was achieved in the online mode. After loading the sample, the enrichment precolumn was flushed with HSA at pH 3.3 for 5 min. Using the ratio of MeCN to aqueous component of 83 17... 

Extreme caution should be used in handling anhydrous HF. It can cause severe bums that may not be noticed immediately but will be very painful later HF dehydrates the skin, and F removes Ca2+from tissues and delays healing. Immediate thorough water washing of any exposed skin should be followed by application of calcium gluconate gel or benzalkonium chloride (trade name Zephiran Chloride), and medical attention is essential. 

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