Visking tubing

The latex was then transferred to 28 mm diameter Visking tubing and a weight of approximately 4kg was placed on the top of the tubing which was immersed in distilled water. After four weeks the volume of the latex was reduced reaching a concentration of 46% w/w. Latex B (which was 4% w/w) was concentrated by rotary evaporation to 28.3% w.w. 

Boc-deprotected branched polypeptide with the Cys(Npys) moiety, poly [Lys(Cyso 2 -DL-Ala3 i)] trifluoroacetate salt (10 mg, 16.5 pmol), was dissolved in 10 mL 0.06 M phosphate buffer (pH 5.5) (see Note 6) 1.35 amount of H-LKNleADPNRFRGKDL-Acp-CSALLEDPVG-NHj (14.8 mg, 5.4 pmol), calculated from the Npys-content of polymer, was added to the reaction mixture. The final concentration was 1 mg/mL for the polymer component. The solution was stirred for 4 h at RT, transferred to a Visking tube (cutoff 8000-12,000), and dialyzed for 2 d against 0.1% acetic acid. No Npys content was detected by UV spectroscopy at A, = 350 nm in the conjugate after freeze drying (see Note 7). 

Place the immunoglobulin solution in Visking tubing and dialyze extensively against several changes of saline to remove sulfate ions. (Alternatively, remove sulfate by chromatography on a Se-phadex G-25 column.)... 

Cdx polymers of different molecular mass were prepared as follows Ep or DiEp was added to the aqueous alkaline solution of cdx. The small molecules (inorganic salt, un-reacted cdx and Ep) were removed from the reaction mixture by dialysis using Visking tubes ( Medicell Int. LTD. The products v ere obtained as white powders by freeze drying. The characteristics of them are given in Table I. Cdx content was determined by iodometry after acid hydrolysis. 

Samples for spectroscopic examination were prepared by treating extremely thin copper(0)/cellulose films supported between Visking tubing (as described elsewherel) with aqueous hexachloroplatinic(IV) acid U.V./visible spectra were recorded on a Pye-Unicam SP8100 spectrophotometer. 

Immersion of copper(0)/cellulose films or filaments in aqueous hexachloroplatinic(IV) acid resulted in an immediate darkening of the brown material after ca. 20 minutes the platinum(0)/cellulose appeared almost black. The very thin samples sandwiched between Visking tubing were a transparent yellow-brown. 

UV-VIS extinction spectra were measitred with a Lambda 35 spectrophotometer (Perkin Elmer, USA). Particle size distribution, based on hydrodynamic diameter, was evaluated by Nano S (Malvern, UK), a dynamic light scattering analyzer (DLS). Urueacted metal cations, extracted from the sample by a semi-permeable osmotic membrane (Visking tube), were detected by ICP-AES qirantitative analysis (Liberty 200, Varian, Austraha) in order to determine the reaction yield. Suspensiorts were dropped and dried on a copper grid, then observed by high resolution transmission electron microscopy (HRTEM) (Tecnai F20) and by the STEM mode with microanalysis EDX. 

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