Vacuolar proteins

P. O. Selgen, P. Bohley, Autophagy and Other Vacuolar Protein Degradation Mechanisms , Experientia 1992, 48, 158-172. 

Amerik, a. Y., Nowak, J., SwAMiNATHAN, S., and Hochstrasser, M. The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell, 2000, 11, 3365-80. 

Finger A, Knop M, Wolf, DH (1993) Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast. Eur J Biochem 218 565-74... 

Nozue, M. et al.. Detection and characterization of a vacuolar protein (VP24) in anthocyanin-producing cells of sweet potato in suspension culture. Plant Cell Physiol, 36, 883, 1995. 

Herman, P.K. Emr, S.D. Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cere-visiae. Mol. Cell. Biol., 10, 6742-6754 (1990)... 

Ahmed SU, Rojo E, Kovaleva V, Venkataraman S, Dombrowski JE, Matsuoka K, Raikhel NV (2000) The plant vacuolar sorting receptor AtELP is involved in transport of NH(2)-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. J Cell Biol 149 1335-1344... 

The treatment with RO fraction slowed down post-imbibition cell development and was directly proportional to the concentration applied. In 1 14-treated roots the protein and lipid reserves were almost completely depleted (Fig. 15.5a). By contrast, in 1 10- and 1 8-roots the vacuolar protein deposits were still abundant, notably in the latter, and lipid reserves appeared to be nearly as abundant as in the 2 h-control roots. 

MCKNIGHT, T.D., ROESSNER, C.A., DEVAGUPTA, R SCOTT, A.I., NESSLER, C.L., Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase from Catharanthus roseus. Nucleic Acids Res., 1990, 18, 4939. 

APS adaptor protein, or BLOC-1-3 (biogenesis of lysosome-related organelle complex), or HOPS (homotypic vacuolar protein sorting or VPS class C complex). Endosomal sorting complex required for transport (ESCRT) including CHMP2B... 

Bonangelino, C. J., Chavez, E. M., Bonifacino, J. S. (2002). Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol. Biol. Cell, 13, 2486-2501. 

Vacuolar protein sorting LUV1, STP22, VPS17, VPS35... 

Paravicini, G., Horazdovsky, B. F., and Emr, S. D. (1992). Alternative pathways for the sorting of soluble vacuolar proteins in yeast Avps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. Mol. Biol. Cell 3, 415-427. 

A family of protein acyltransferases (PATs) is responsible for S-acylation of proteins in cells (S. Lobo, 2002 A. Roth, 2002) [8]. Members of this family are characterized by the presence of a cysteine-rich domain containing a DHHC (Asp-His-His-Cys) motif. PATs are polytopic membrane proteins with the putative catalytic DHHC motif localized to a cytoplasmic loop between transmembrane spans. Some PATs function alone whereas others, such as the yeast Ras PAT Erf2, require a cytoplasmic protein, Erf4, for activity. It is likely that particular classes of substrate have a dedicated PAT that accounts for most, if not all, of their S-acylation. For example, Swflp modifies SNARE proteins and other monotopic membrane proteins with a juxtamembrane cysteine residue. The yeast vacuolar protein Vac8 is mainly S-acylated by the vacuolar DHHC protein Pfa3 (J.E. Smotrys,... 

Sortinl affects the vacuolar trafficking of both proteins and flavonoids, indicating that flavonoid transport to the vacuole could share the same membrane-mediated pathway as vacuolar proteins. But this does not rule out the possibility that one of the trafficking defects is direct, whereas the other one is indirect. The fact that Sortinl mimics BSO to produce an oxidized cell environment to affect ABC transporter activity supports a model that a transporter-mediated flavonoid transport defect induces miss-trafficking of other vacuolar contents such as the proteins. On the other hand, mutants defective in flavonoid metabolism, such as tt4, show hypersensitivity to Sortinl and have altered vacuolar morphology... 

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