Cysteine-rich domain

The major surface coat component of Toxocara larvae runs as a set of four closely migrating bands with apparent mobility of 120 kDa on SDS-PAGE. One of these was cloned and sequenced, identified as a serine-rich mucin and designated MUC-1 (Gems and Maizels, 1996). We have now established that there are at least five distinct mucin genes in this parasite, which bear general similarity but important distinctions. Thus, MUC-2, MUC-3, MUC-4 and MUC-5 are all threonine-rich rather than serine-rich, and all five differ in the repeat motifs within the mucin domains. All have similar non-mucin, cysteine-rich domains originally termed NC6 (nematode six-cysteine) domains, and since renamed SXC (six-cysteine), as described below. All mucins have a pair of SXC domains at their C-terminus, while MUC-3 and MUC-5 also have paired N-terminal SXC domains. 

Hu, J Reyes-Cruz, G., Goldsmith, R K., and Spiegel, A. M. (2001) The Venus s-flytrap and cystein-rich domains of the human Ca2+ receptor are not linked by disulfide bonds. J. Biol. Chem. 276,6901-6904. 

Cl Protein kinase C conserved region 1 (Cl) domains (cysteine-rich domains) E(MFP) 1(2) 35(53) 1FAQ... 

The second type of aberrant Fas, designated as the membrane-binding decorative receptor, which has substitutions of amino acid(s) within the EC domain, leading to an alteration of the conformation of Fas-ligand binding site (Bl), especially in cysteine-rich domains, can be anchored in the cell membrane. However, this... 

Asp, Glu - rich domain immungiobuiin-iike domain Cysteine rich domain... 

ErbB receptors exhibit conserved structural similarity and domain topography (Fig. 1). They contain an extracellular ligand-binding region, which consists of two cysteine-rich domains, and is responsible for ligand-induced receptor dimerization. This... 

B) Electron micrographs of single molecules of laminin. (A) and (B) from Yurchenko and Schittny.663 663a Courtesy of Peter Yurchenko (C) Structural model of the three-chain laminin molecule with domain designations. From Beck et al.670 Domains I and II are a triplehelical coiled coil rod which, in the Bl chain, is interrupted by a small cysteine-rich domain a. Sites of cleavage by cathepsin are marked by arrows. 

Shinshi, H., Neuhaus, J.M., Ryals, J. Meins, F. (1990). Structure of a tobacco endochitinase gene evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Molecular Biology 14, 357-68. 

Bies, R. D., Caskey, C. T., and Fenwick, R. (1992). An intact cysteine-rich domain is required for dystrophin function. J. Clin. Invest. 90, 666-672. 

Suzuki, A., Yoshida, M., Yamamoto, H., and Ozawa, E., 1992, Glycoprotein-binding site of dystrophin is confined to the cysteine-rich domain and the first half of the carboxy-terminal domain, FEBS Lett, 308, pp 154-160. 

Fig. 2.1 Multi-domain structural organization of gastrointestinal gel-forming mucins. Schematic drawings (not at scale) of the different domains found in gastrointestinal mucins and their roles. Note that while the cysteine-rich domains (D-, CS-, C-, and CK-domains) have a similar length among mucin alleles, the O-glycosylated regions, except in the case of MUC5B, differ in size, a feature not shown in the drawings...

D-domain, which contains up to 30 cysteines and up to 400 amino acid residues, shows significant sequence identity with the other D-domains, especially the cysteine, glycine, and proline residues. Further analysis of the D-domain sequences has defined a potential domain, known as the trypsin inhibitor-like cysteine-rich domain (TIL) within each D-domain sequence (Lang et al. 2004). The NH2-terminal D-domains are involved in formation of mucin disulfide-linked oligomers/multimers (Perez-Vilar and Hill 1999 Perez-Vilar and Mabolo 2007 see Section 2.3.2). 

Thim L., Madsen F. and Poulsen S.S. (2002) Effect of trefoil factors on the viscoelastic properties of mucus gels. Eur J Clin Invest 32, 519-527 Tomasetto C., Masson R., Linares J.L., Wendling C., Levebvre O. and Chenard M.P. (2000) pS2/TFFl interacts directly with the VWFC cysteine-rich domains of mucins. Gastroenterology 118, 70-80... 

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