DNA replication in vitro

Schwartz CS, Snyder R, Kalf GF. 1985. The inhibition of mitochondrial DNA replication in vitro by the metabolites of benzene, hydroquinone and p-benzoquinone. Chem-Biol Interact 53 327-350. 

Smith S, Stillman B (1991) Stepwise assembly of chromatin during DNA replication in vitro. EMBO J 10 971-980... 

Past-active In some recent studies,... Cr + increased DNA polymerase processivity and decreased its fidelity during DNA replication in vitro (13-17). (Adapted from Plaper et ak, 2002)... 

The Cadet laboratory has prepared phosphoramidites of stereoisomers for 5, 8-cyclo-dA and 5, 8-cyclo-dG for incorporation into oligonucleotides. Primer extension assays using the mammalian replicative enzyme pol 8 demonstrated that 5, 8-cyclonucleosides block DNA replication in vitro and thus would be highly... 

Prelich, G, Kostura, M., Marshak, D R, Matthews, M B., and Stillman, B. (1987) The cell-cycle regulated proliferating cell nuclear antigen is required for SV40-DNA replication in vitro Nature 326,471-475... 

Taylor, R.W., Chinnery, P.P., Turnbull, D M. and Lightowlers, R.N. (1997) Selective inhibition of mutant human mitochondrial DNA replication in vitro by peptide nucleic acids. Nature Genetics, 15, 212-215. 

Snow ET, Xu -SL. 1989. Effects of chromium(III) on DNA replication in vitro. Biological Trace Element Res 21 61-71. 

DNA replication in vitro using a range of replicative and translesion polymerases. 

Alekseyev, Y.O. and Romano, LJ. (2002) Effects of benzo[a]pyrene adduct stereochemistry on downstream DNA replication in vitro evidence for different adduct conformations within the active site of DNA polymerase I (Klenow fragment). Biochemistry, 41, 4467-4479. 

Bullock, P. A. 1997. The initiation of simian virus 40 DNA replication in vitro. Crit. Rev. Biochem. Mol. Biol. 32 503-568. 

Eukaryotic DNA polymerases have also been isolated and characterized as listed in Table 22.1. Based on studies of SV40 DNA replication in vitro, it has been found that DNA polymerase d has high processivity and is required for leading-strand synthesis, making it analogous to E. coli DNA pol III. DNA polymerase d requires ATP and is stimulated by two additional DNA replication proteins, RF-C and PCNA. DNA polymerase a serves the same role as E. coli DNA pol I in that DNA polymerase a is necessary for lagging-strand synthesis. In addition to DNA polymerase a and d, three other DNA polymerizing activities have been identified. DNA polymerase I is involved in DNA repair and is most similar to E. coli DNA pol II. DNA polymerase b is also a repair enzyme, and DNA polymerase g is required for mitochondrial DNA synthesis. 

Snow ET (1994) Effects of chromium on DNA replication in vitro. Environ Health Perspect 102, Suppl 3 41-44. 

Higuchi, K., Katayama, T., Iwai, S., Hidaka, M., Horiuchi, T., and Maki, H. (2003). Fate of DNA replication fork encountering a single DNA lesion during oriC plasmid DNA replication in vitro. Genes Cells 8, 437 49. 

Challberg, M. D., Desiderio, S. V., and Kelly, T. J., 1980, Adenovirus DNA replication in vitro Characterization of a protein covalently linked to nascent DNA strands, Proc. Natl., Acad. Sci. USA 77 5105. 

Enomoto, T., Lichy, J. H., Ikeda, J. E., and Herwitz, J., 1981, Adenovirus DNA replication in vitro Purification of the terminal protein in a functional form, Proc. Natl. Acad. Sci. USA 78 6779. 

Nagata, K., Guggenheimer, R. A. and Hurwitz, J., 1983, Adenovirus DNA replication in vitro Synthesis of full length DNA with purified proteins, Proc. Nat. Acad. Sci. USA 80 4266. 

Gautschi, J. R., Burkhalter, M., and Reinhard, P., 1977, Semiconservative DNA replication in vitro. II. Replicative intermediates of mouse P-815 cells, Biochim. Biophys. Acta 474 512. 

A second possibility to account for the observed defect in DNA synthesis during growth at low unsaturated acid levels is that the decreased fluidity of the membrane under these conditions directly affects the process of mitochondrial DNA replication. It has been suggested that mitochondrial DNA synthesis is a membrane-associated process. Recent work in our laboratory has demonstrated that the fluidity of the mitochondrial membrane has a direct influence on mitochondrial DNA replication in vitro An Arrhenius plot of DNA replication in vitro has a discontinuity (Fig. 5), the temperature of which varies with the unsaturated fatty acid content of the mitochondrial membranes (Table II). These results demonstrate the existence of a close functional relationship between mitochondrial DNA replication and the physical properties of the mitochondrial membrane. 

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