Urine protein profiling

Hampel, D. J., C. Sansome, M. Sha, S. Brodsky, W. E. Lawson, and M. S. Goligorsky. 2001. Towards proteomics in uroscopy Urine protein profiles after radiocontrast medium administration. Journal of the American Society of Nephrology 12 1026-1035. 

For a summary of available protocols for urine protein profiling, see Table 3. 

Schaub, S., Wilkins, J., Weiler, T., Sangster, K., Rush, D., and Nickerson, P. Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry. Kidney Int, 65 323-332, 2004. 

Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry. Kidney Int. 65, 323-332. 

The use of proteomics in prostate cancer, the most common cancer in men, was reviewed [94]. In vitro studies on prostate cell lines provided positive proof-of-principle results in the identification of novel biomarker when proteomics was utilized to query prostate tissue specimens. 2D-PAGE followed by MS was used to investigate protein profiles in voided urine after prostatic massage in patients with prostate cancer or with benign prostatic hyperplasia [95]. In this study, a potential novel cancer biomarker, calgranulin B/MRP-14, was identified. In another study, agarose 2D-gel... 

Protein Chip technology, it will be possible to simultaneously analyze protein profiles of body fluids such as serum and urine samples very rapidly. The SELDI mass spectrometry in conjunction with bioinformatics tools could greatly facilitate the discovery of new and improved toxicologic biomarkers. 

Forterre, S., J. Raila, and F. J. Schweigert. 2004. Protein profiling of urine from dogs with renal disease using ProteinChip analysis. Journal of Veterinary Diagnostic Investigation 16 271-277. 

An investigation of several methodological aspects of obtaining urinary protein profiles by SELDI-TOF-MS revealed that among the extrinsic factors instrument settings and matrix composition critically influenced peak detection and reproducibility, while freeze-thaw cycles had minimal effects. Intrinsic factors of significance included blood in urine, dilution, and first-void vs. midstream urine [100]. 

In common with M. macedonicus, urine from male M. spretus also demonstrated a MUP-sized band following gel electrophoresis. The proteins within this band were analysed by high resolution anion exchange chromatography and electrospray ionisation mass spectrometry (ESI-MS). The former technique produced an elution profile consisting of just three peaks, in contrast to both the more complex patterns observed previously from M. m. domesticus and the single major peak in M. macedonicus. Furthermore, similar analyses from five individual males resulted in near... 

Meloxicam, a COX-2 inhibitor, has been marketed in some countries and promoted as having an improved safety profile over current NSAIDs. It has a long half-life, is highly protein-bound, is metabolized to inactive compounds, and is excreted in the urine and feces. 

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