Uridine-5 -diphosphate-a-d-glucose

Figure 4 Representative NMR spectra (161.93 MHz) of sucrose-supplied and sucrose-deprived (120 h) sycamore cells. Cells harvested from the culture medium were rinsed three times by successive resuspensions in fresh culture medium devoid of sucrose and incubated at zero time (20 mg wet weight / ml) into flasks containing sucrose-free culture medium. Cells (normal cells and 120-h sucrose starved cells (9 g) were harvested for in vivo examination. The spectra recorded at 20 C with a 50° pulse angle and a 0.6-s repetition time were the result of 6000 transients (1 h). Perfusion medium A, standard culture medium B, culture medium devoid of sucrose since 120 h. Peak assigments Glc-6-P, glucose-6-P Fru-6-P, fhictose-6-P Cyt-pi, cytoplasmic phosphate (at pH 7.5) vac-Pi, vacuolar phosphate (at pH 5.7) NTP, nucleotides triphosphate UDPG, uridine-5 -diphosphate-a-D-glucose...

UDP-glucose, uridine 5 -diphosphate a-D-glucopyranosyl ester UMP, uridine 5 -monophosphate UTP, uridine 5 -triphosphate... 

Synthesis of sucrose 6 -phosphate by an enzymic method using uridine 5 -(a-D-glucopyranosyl diphosphate) plus D-glucose 6-phosphate has been reported.126-131 The first, unambiguous, chemical synthesis of sucrose 6 -phosphate was achieved by Buchanan and coworkers.18 The reaction of 2,3,4,6,l, 3, 4 -hepta-0-acetylsucrose, prepared by five steps of synthesis, with cyanoethyl phosphate in pyridine gave a crude product from which sucrose 6 -phosphate was isolated as the barium salt. 

B. Glycosidation UD P-glucosyltransferase As above to a limited extent RXH + uridine diphospho- 8-D-glucose RX-glucoside + uridine-5 -diphosphate... 

The intravenous injection of 2-amino-2-deoxy-D-glucose-14C into rats, and subsequent isolation of the uridine diphosphate N-acetyl-hexosamine fraction, has been suggested as a fast and convenient method for preparing small amounts of a mixture of radioactive uridine 5 -(2-acetamido-2-deoxy-a-D-gIucopyranosyl pyrophosphate) and uridine 5 -(2-acetamido-2-deoxy-a-D-galactopyranosyl pyrophosphate). 

Phosphorolysis of ribonucleic acid with polynucleotide phosphorylase gives a mixture of the diphosphates of the four common nucleosides, which are transformed into triphosphates with enolpyruvate phosphate and pyruvate kinase. This mixture may be used as such as a source of uridine triphosphate in the preparation of the nucleotide-sugar uridine 5 -(a-D-glucopy-ranosyl diphosphate) ( uridine-diphosphate-glucose, UDP-Glc), or as a... 

At present, it seems that uridine 5-(D-glucosyluronic acid pyrophosphate) is formed only through the oxidation of uridine diphosphate D-glucose, Attempts to join either a- or /3-n-glucosyluronate phosphate to uridine 5-(trihydrogen pyrophosphate) through the reversal of pyrophosphorolysis,... 

L. is the most important carbohydrate in the milk of all mammals. Human milk contains 6-8%, cow s milk 4-5%. L. is a component of a few oligosaccharides, and also occurs in plants, e.g. in fruits and pollen. In L. synthesis in the milk glands of mammals, the galactose residue of uridine diphosphate p-galac-tose is transferred and linked to the OH on C4 of d-glucose, a process catalysed by lactose synthetase,... 

Uridine 5 -(trihydrogen diphosphate) mono-a-D-glucopyranosyl ester, 9CI. UDPG. UDP Glucose [133-89-1]... 

The glycosyl phosphate (6l) has been synthesized from 2-amino-2-deoxy-D-glucose by standard methods, and condensed with appropriate nucleoside 5 -phosphate derivatives to give cytldine, uridine, and adenosine 5 -(2,6-dlamlno-2,6-dldeoxy-a-D-glucopyranosyl diphosphates) which are potential biosynthetic intermediates for... 

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