Uridine, 5- diphosphate D-glucose

Starch synthetase Adenosine/Uridine diphosphate D-glucose -... 

At present, it seems that uridine 5-(D-glucosyluronic acid pyrophosphate) is formed only through the oxidation of uridine diphosphate D-glucose, Attempts to join either a- or /3-n-glucosyluronate phosphate to uridine 5-(trihydrogen pyrophosphate) through the reversal of pyrophosphorolysis,... 

It is not yet known whether uridine diphosphate AT-acetyl-n-glucos-amine can be formed by a transfer reaction between AT-acetyl-n-glucos-amine and uridine diphosphate D-glucose. ... 

Flowers, H.M., Batra, K.K., Kemp, J. Hassid, W.Z. (1968) Biosynthesis ot insoluble Glucans from Uridine Diphosphate-D-glucose with Enzyme Preparations from Phaseolus aureus and Lupinus albi, Plant Physiology, 43, 1703-9 Forsee, W.T., Griffin, J.A. Schutzbach, J.S. (1977) Mannosyltransfer fi om GDP-Mannose to Oligosaccharide Lipids , Biochemical and Biophysical Research Communications, 75, 799-805... 

From extensive analysis of recombinant proteins, and the crystal structure of A. thaliana protein, detailed reaction mechanisms have been proposed. The ANS reaction likely proceeds via stereospecific hydroxylation of the leucoanthocyanidin (flavan-3,4-cA-diol) at the C-3 to give a flavan-3,3,4-triol, which spontaneously 2,3-dehydrates and isomerizes to 2-flaven-3,4-diol, which then spontaneously isomerizes to a thermodynamically more stable anthocyanidin pseudobase, 3-flaven-2,3-diol (Figure 3.2). The formation of 3-flaven-2,3-diol via the 2-flaven-3,4-diol was previously hypothesized by Heller and Forkmann. The reaction sequence, and the subsequent formation of the anthocyanidin 3-D-glycoside, does not require activity of a separate dehydratase, which was once postulated. Recombinant ANS and uridine diphosphate (UDP)-glucose flavonoid 3-D-glucosyltransferase (F3GT, sometimes... 

Synthesis of sucrose 6 -phosphate by an enzymic method using uridine 5 -(a-D-glucopyranosyl diphosphate) plus D-glucose 6-phosphate has been reported.126-131 The first, unambiguous, chemical synthesis of sucrose 6 -phosphate was achieved by Buchanan and coworkers.18 The reaction of 2,3,4,6,l, 3, 4 -hepta-0-acetylsucrose, prepared by five steps of synthesis, with cyanoethyl phosphate in pyridine gave a crude product from which sucrose 6 -phosphate was isolated as the barium salt. 

The intravenous injection of 2-amino-2-deoxy-D-glucose-14C into rats, and subsequent isolation of the uridine diphosphate N-acetyl-hexosamine fraction, has been suggested as a fast and convenient method for preparing small amounts of a mixture of radioactive uridine 5 -(2-acetamido-2-deoxy-a-D-gIucopyranosyl pyrophosphate) and uridine 5 -(2-acetamido-2-deoxy-a-D-galactopyranosyl pyrophosphate). 

Phosphorolysis of ribonucleic acid with polynucleotide phosphorylase gives a mixture of the diphosphates of the four common nucleosides, which are transformed into triphosphates with enolpyruvate phosphate and pyruvate kinase. This mixture may be used as such as a source of uridine triphosphate in the preparation of the nucleotide-sugar uridine 5 -(a-D-glucopy-ranosyl diphosphate) ( uridine-diphosphate-glucose, UDP-Glc), or as a... 

Stoichiometric quantities of uridine diphosphate glucose were used, in the presence of a transfer enzyme, sucrose synthetase, in the soluble state (extraction given). Coupling with modified D-fructose gave sucroses modified on the D-fructosyl group, on the 1 -3-mmol scale. Thus were prepared l -deoxy-l -fluoro- (59%),98 4 -deoxy-4 -fluoro- (16%), and l -azido-l -deoxy-sucrose (15%).71 6-Deoxy-6-fluoro-D-glucose was isomerized to 6-deoxy-6-fluoro-D-fructose with isomerase, and gave 6 -deoxy-6 -fluoro-sucrose.71... 

E. I. Budowsky, T. N. Druzhinina, G. I. Eliseeva, N. D. Gabrielyan, N. K. Kochetkov, M. A. Novikova, V. N. Shibaev, and G. L. Zhdanov, Synthetic analogues of uridine diphosphate glucose Biochemical and chemical studies. The secondary structure of nucleoside diphosphate sugars, Biochim. Biophys. Acta, 122 (1966) 213-224. 

---