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Uninfected E. coli

The hydroxymethylase activity cannot be detected in uninfected E. coli. It may be noted that this reaction differs from thymidylate synthesis in that a reduction is not involved, as indicated by the fact that H -folate is a product. In studies of the mechanism of the reaction catalyzed by the the T4-induced hydroxymethylase, Yeh and Greenberg IS) have shown that tritium is displaced from deoxycytidylate-5- H with the formation of product. As well, the enzyme catalyzed an H4-folate-dependent exchange between water and the hydrogen atom at the 5-position of dCMP. [Pg.234]

Experiments using a coupled transcription-translation system from uninfected E, coli instead of a pure transcription system, led to the same conclusion. Under conditions which did not allow the activation of phage RNA polymerase, E. coli RNA polymerase transcribed the lysozyme gene (Herrlich and ScHWEiGER, 1970), but apparently terminated RNA synthesis thereafter because no late proteins were detected (unpublished). [Pg.66]

Huang and Ochoa, 1972). The significance of such an interference factor and of changes in IF3 activity to T4 infection is not yet established. An explanation for translation control in T4 infection must account for the fact that "late" proteins of T4 can be synthesized in vitro using late T4 mRNA and ribosomes isolated from uninfected E. coli (Salser et al., I967) and that the time of IF3 inactivation measured so far is too late for an involvement in the host shut-off. [Pg.79]

Escherichia coli (strain B [28] overproducing strain JM83(pKT8P3) [31] infected and uninfected [32] infected by bacteriophage T4, host-coded enzyme from infected E. coli is part of bacteriophage T4 dNTP-synthesiz-ing multi-enzyme complex [25]) [25, 28, 31, 32]... [Pg.522]

That the development of bacterial infections in iron-treated animals is caused by the alleviation of iron-starvation for infecting bacteria rather than by the neutralization of defense mechanisms by iron has been shown most clearly in siderophore-treated animals. Treatment of mice with enterochelin produced no observable effect in uninfected mice whereas in infected mice, E. coli grew logarithmically, and the animals died within 18 hr. This iron-binding compound facilitated the development of the overwhelming infection by being able to remove iron from Tr (8). [Pg.79]

To focus on this reduced usage of CpLFpG following infection, an in vitro system was set up to study any differential uptake into protein of leucine from different tRNA subspecies in response to natural mRNA. The system, composed of polysomes, polysomal tRNA obtained from uninfected and T2-infected E. coli and charged with [ Hjleucine and 19 other unlabeled amino acids, [ C]Leu-tRNAi, and an E. coli aminoacyl-tRNA synthetase preparation, incorporated leucine into protein at a relatively high efficiency. [Pg.163]

Sueoka and Kano-Sueoka (1964) studied the biochemical and physico-chemical properties of 17 forms of transfer RNA (sRNA) of E, coli after infection with T2 phage. For 16 forms of aminoacyl-sRNA no difference was found between their properties in uninfected and infected cells, but with leucyl-sRNA fractions obtained at various times after infection, appreciable differences were present. In the period between 3 and 5 min after infection a new peak of leucyl-sRNA appeared, to disappear again at 8 min. Allowing for degeneracy of the code, it can be assumed that the new peak contains a leucine anticodon differing from the leucine anticodon in the sRNA of E. coli. According to code deciphering experiments, several codons in the template can code leucine (CUG, CUC, CUU, UUA, UUC). [Pg.59]


See other pages where Uninfected E. coli is mentioned: [Pg.326]    [Pg.135]    [Pg.318]    [Pg.258]    [Pg.97]    [Pg.373]    [Pg.74]    [Pg.79]    [Pg.326]    [Pg.135]    [Pg.318]    [Pg.258]    [Pg.97]    [Pg.373]    [Pg.74]    [Pg.79]    [Pg.122]    [Pg.220]    [Pg.487]    [Pg.953]    [Pg.71]    [Pg.2562]    [Pg.156]    [Pg.302]    [Pg.87]    [Pg.15]    [Pg.186]    [Pg.247]    [Pg.255]    [Pg.124]    [Pg.79]   
See also in sourсe #XX -- [ Pg.74 , Pg.79 ]




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E. coli

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