Lysozyme gene

LysS/LysE an additional chloramphenicol resistant plasmid carries the gene forT7 lysozyme under constitutively active promoters. T7 lysozyme inhibits the activity of T7 polymerase thereby reducing basal (uninduced) polymerase activity/ protein expression. LysE express higher levels of T7 lysozyme for tighter control. pLacI an additional chloramphenicol resistant plasmid carries the gene for high level production of the lac repressor to reduce basal expression. 

Base-pairs in the gene, needed to code for the enzyme lysozyme (129 amino acids) found in egg white. 

Many bacterial enzymes and proteins, which are modified by the introduction of F-Phe or F-Tryp, have been obtained. Mammalian proteins containing F-Pro or other fluorinated amino acids have also been obtained, either in a direct manner in vivo) or, more efficiently, by the expression of the gene in a bacteria. Thus, trifluoro-methionine has been incorporated by E. coli in the lysozyme of a bacteriophage. Because this enzyme contains three methionines, it has been used to study the interactions of this protein with its ligands by F NMR. ... 

Pita, J. C., Howell, D. S., Kuettner, K. Evidence for a role of lysozyme in endochondral calcification during healing of ricketts. In Extracellular matrix influence on gene expression. Slavkin, H. C., Greulich, R. E. (eds.). New York Academic Press 1975... 

Use the BLAST tool to compare the amino acid sequences for human a-lactalbumin and lysozyme. Repeat the process using BLAST to compare the nucleotide sequences for the genes coding for human a-lactalbumin and lysozyme. 

Figure 9.3. Restriction map produced by Webcutter. The partial restriction map shows the nucleotide sequence of human lysozyme gene submitted to Webcutter using options for all restriction endonucleases with recognition sites equal to or greater than six nucleotides long and cutting the sequence 2 6 times (at least 2 times and at most 6 times). The restriction profile (map) is returned if Map of restriction sites is selected for display. The tables by enzyme name and by base pair number can be also returned if displays for Table of sites, sorted alphabetically by enzyme name Table of sites, sorted sequentially by base pair number are chosen.

Figure 10.8. Gene identification by Procrustes. The nucleotide sequence encoding human lysozyme is used as a query sequence to identify its gene structure against known protein sequence (i.e., pig lysozyme protein). The output includes sequence aignment of the source (predicted translate) versus target protein (pig lysozyme).

Figure 10.11. Outputs from FGENESH and FGENE of GeneFinder. The nucleotide sequence of DNA encoding human lysozyme (5648 bp) is submitted to Sanger Centre for gene identification with FGenesh (hidden Markov model) and Fgene (pattern recognition) tools. The identical translate is predicted by the two programs.

It has now been found that the glucocorticoid response elements of mouse mammary tumour virus and the gene for chicken lysozyme also bind progesterone re-... 

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