Unfolding of protein

Klotz, I. M., 1996. Equilibrium constants and free energies in unfolding of proteins in urea solutions. Proceedings of the National Academy of Sciences 93 14411-14415. 

K. Benedek, S. Dong and B. E. Kaiger, Kinetics of unfolding of proteins on hydrophobic surfaces in reversed-phase liquid chiomatography , /. Chromatogr. 317 227-243 (1984). 

Hermans, Jr., J., Lohr, D. and Ferro, D. Treatment of the Folding and Unfolding of Protein Molecules in Solution According to a Lattic Model. Vol. 9, pp. 229-283. 

Degradation. There is, however, a second, more important, feature. Above the transition temperature we have shown that both xanthan and succinoglycan are more susceptible to degradation (5). This may be because the polymer side chains become dissociated from the main chain, in a similar manner to the unfolding of proteins. Viscosity changes, however, indicate that main chain linkages have become more... 

Raman optical activity has proved valuable in recent studies of the structure and behavior of a number of natively unfolded proteins found in quite different biological situations. Although completely unfolded, the structures appear to be more stable than those generated by complete unfolding of proteins having a tertiary fold in their native states. Such natively unfolded proteins clearly have special characteristics built into their amino acid sequences that prevent aggregation under normal physiological conditions. 

Garcia, A.E. Onuchic, J.N., Folding a protein in a computer An atomic description of the folding/unfolding of protein A, Proc. Natl Acad. Sci. USA 2003,100, 13898-13903... 

L. Konermann and D. J. Douglas. Unfolding of Proteins Monitored by Electrospray Ionization Mass Spectrometry A Comparison of Positive and Negative Ion Modes. J. Am. Soc. Mass Spectrom., 9(1998) 1248-1254. 

Y Ishihama, Y Oda, N Asakawa, M Iwakura. Nanoscale monitoring of the thermally induced unfolding of proteins using capillary electrophoresis with in-column incubation. Anal. Sci. 13 931-938 (1997). 

Figure 6. PuUing single molecules, (a) Stretching DNA (b) unzipping DNA (c) mechanical unfolding of RNA, and (d) mechanical unfolding of proteins. (See color insert.)...

Unfolding of protein molecules is an endothermic process that can... 

The thermal unfolding of proteins is best measured by differential scanning calorimetry, which measures the heat absorbed by a protein as it is slowly heated through its melting transition (Figure 17.1). A solution of about 1 mg of protein in 1 mL of buffer and a separate reference sample of buffer alone are heated electrically.6 The additional current required to heat the protein solution is recorded. As the protein denatures, there is a large uptake of heat because the process is highly endothermic. The temperature at the maximum of the peak is... 

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